Particle interactions in a magnetophoretic system
<p>The continuous flow separation of magnetic particles from a mixture of particles could improve the performance of magnetic bead based assays but the formation of agglomerates limit the separation efficiency. Bead agglomerates are formed as a result of magnetic binding forces while the hydro...
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| Format: | Thesis |
| Language: | English |
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2016
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| _version_ | 1797102931909017600 |
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| author | Oduwole, O |
| author2 | Sheard, S |
| author_facet | Sheard, S Oduwole, O |
| author_sort | Oduwole, O |
| collection | OXFORD |
| description | <p>The continuous flow separation of magnetic particles from a mixture of particles could improve the performance of magnetic bead based assays but the formation of agglomerates limit the separation efficiency. Bead agglomerates are formed as a result of magnetic binding forces while the hydrodynamic fluid environment strongly influences their movement. The ability to predict the interaction between nearby beads will help to determine a threshold separation distance which will be recommended for use when obtaining measurement within a magnetic bead assay for a specified time interval.</p> <p>The introductory part of this thesis explored the development of a two dimensional numerical model in Matlab which predicts the trajectory pattern as well as magnetic induced velocities between a pair of super-paramagnetic beads suspended in water within a uniform field.</p> <p>The movement of a bead pair interacting due to both magnetic and hydrodynamic forces within a magnetophoretic system was recorded using an optical system; the beads’ movements were compared with the simulated trajectories and gave a good agreement. The model was used to predict the shortest agglomeration time for a given separation distance which is of practical benefit to users of bead based assays.</p> <p>The concluding part of this thesis expanded the simulation into a three dimensional model to predict the interactions among three super-paramagnetic beads within a magnetophoretic system. In order to determine the height of the magnetic beads, a Huygens-Fresnel model was implemented in Matlab which was compared with off-focused diffracted images of the beads viewed under an optical system. A good comparison was obtained by comparing the simulated three-dimensional trajectories with experimental data.</p> |
| first_indexed | 2024-03-07T06:12:51Z |
| format | Thesis |
| id | oxford-uuid:f01cbb33-4dd4-4057-8891-7097e6493bce |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:12:51Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | oxford-uuid:f01cbb33-4dd4-4057-8891-7097e6493bce2022-03-27T11:45:21ZParticle interactions in a magnetophoretic systemThesishttp://purl.org/coar/resource_type/c_db06uuid:f01cbb33-4dd4-4057-8891-7097e6493bceEngineeringBiomedical EngineeringEnglishORA Deposit2016Oduwole, OSheard, S<p>The continuous flow separation of magnetic particles from a mixture of particles could improve the performance of magnetic bead based assays but the formation of agglomerates limit the separation efficiency. Bead agglomerates are formed as a result of magnetic binding forces while the hydrodynamic fluid environment strongly influences their movement. The ability to predict the interaction between nearby beads will help to determine a threshold separation distance which will be recommended for use when obtaining measurement within a magnetic bead assay for a specified time interval.</p> <p>The introductory part of this thesis explored the development of a two dimensional numerical model in Matlab which predicts the trajectory pattern as well as magnetic induced velocities between a pair of super-paramagnetic beads suspended in water within a uniform field.</p> <p>The movement of a bead pair interacting due to both magnetic and hydrodynamic forces within a magnetophoretic system was recorded using an optical system; the beads’ movements were compared with the simulated trajectories and gave a good agreement. The model was used to predict the shortest agglomeration time for a given separation distance which is of practical benefit to users of bead based assays.</p> <p>The concluding part of this thesis expanded the simulation into a three dimensional model to predict the interactions among three super-paramagnetic beads within a magnetophoretic system. In order to determine the height of the magnetic beads, a Huygens-Fresnel model was implemented in Matlab which was compared with off-focused diffracted images of the beads viewed under an optical system. A good comparison was obtained by comparing the simulated three-dimensional trajectories with experimental data.</p> |
| spellingShingle | Engineering Biomedical Engineering Oduwole, O Particle interactions in a magnetophoretic system |
| title | Particle interactions in a magnetophoretic system |
| title_full | Particle interactions in a magnetophoretic system |
| title_fullStr | Particle interactions in a magnetophoretic system |
| title_full_unstemmed | Particle interactions in a magnetophoretic system |
| title_short | Particle interactions in a magnetophoretic system |
| title_sort | particle interactions in a magnetophoretic system |
| topic | Engineering Biomedical Engineering |
| work_keys_str_mv | AT oduwoleo particleinteractionsinamagnetophoreticsystem |