Joint genotype calling with array and sequence data.
Analysis of rare variants is currently a major focus of genetic studies of human disease. Single-nucleotide polymorphism (SNP) genotypes can be assayed using microarray genotyping or by sequencing, but neither technology produces perfect genotype calls, especially at rare SNPs. Studies that collect...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826304138029301760 |
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| author | O'Connell, J Marchini, J |
| author_facet | O'Connell, J Marchini, J |
| author_sort | O'Connell, J |
| collection | OXFORD |
| description | Analysis of rare variants is currently a major focus of genetic studies of human disease. Single-nucleotide polymorphism (SNP) genotypes can be assayed using microarray genotyping or by sequencing, but neither technology produces perfect genotype calls, especially at rare SNPs. Studies that collect both types of data are becoming increasingly common, so it may be possible to combine data types to increase accuracy. We present a method, called Chiamante, which calls genotypes on individuals with either array data, sequence data, or both. The model adapts to data quality and can estimate when either the array or the sequence data should be ignored when calling the genotypes at each SNP. As a special case, our method will call genotypes from only array data and outperforms existing methods in this scenario. We have applied our method to array and sequence data from Phase I of the 1000 Genomes Project and show that it provides improved performance, especially at rare SNPs. This method provides a foundation for future efforts to fuse genetic data from different sources, for example, when combining data from exome sequencing and exome microarrays. |
| first_indexed | 2024-03-07T06:13:15Z |
| format | Journal article |
| id | oxford-uuid:f03d7174-07f8-4bf2-9065-0c7492e35dfa |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:13:15Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:f03d7174-07f8-4bf2-9065-0c7492e35dfa2022-03-27T11:46:20ZJoint genotype calling with array and sequence data.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f03d7174-07f8-4bf2-9065-0c7492e35dfaEnglishSymplectic Elements at Oxford2012O'Connell, JMarchini, JAnalysis of rare variants is currently a major focus of genetic studies of human disease. Single-nucleotide polymorphism (SNP) genotypes can be assayed using microarray genotyping or by sequencing, but neither technology produces perfect genotype calls, especially at rare SNPs. Studies that collect both types of data are becoming increasingly common, so it may be possible to combine data types to increase accuracy. We present a method, called Chiamante, which calls genotypes on individuals with either array data, sequence data, or both. The model adapts to data quality and can estimate when either the array or the sequence data should be ignored when calling the genotypes at each SNP. As a special case, our method will call genotypes from only array data and outperforms existing methods in this scenario. We have applied our method to array and sequence data from Phase I of the 1000 Genomes Project and show that it provides improved performance, especially at rare SNPs. This method provides a foundation for future efforts to fuse genetic data from different sources, for example, when combining data from exome sequencing and exome microarrays. |
| spellingShingle | O'Connell, J Marchini, J Joint genotype calling with array and sequence data. |
| title | Joint genotype calling with array and sequence data. |
| title_full | Joint genotype calling with array and sequence data. |
| title_fullStr | Joint genotype calling with array and sequence data. |
| title_full_unstemmed | Joint genotype calling with array and sequence data. |
| title_short | Joint genotype calling with array and sequence data. |
| title_sort | joint genotype calling with array and sequence data |
| work_keys_str_mv | AT oconnellj jointgenotypecallingwitharrayandsequencedata AT marchinij jointgenotypecallingwitharrayandsequencedata |