Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice.
Excessive accumulation of Ca2+ in neurones and glutamate release are involved in neuropathological processes, including ischaemia. We investigated the neuroprotective effects of the Ca2+ channel antagonist, omega-Aga-IVA, in CA1 pyramidal neurones in rat hippocampal slices following an in vitro hypo...
| Main Authors: | , , , , |
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| 格式: | Journal article |
| 语言: | English |
| 出版: |
1995
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| _version_ | 1826304168603680768 |
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| author | Small, D Monette, R Mealing, G Buchan, A Morley, P |
| author_facet | Small, D Monette, R Mealing, G Buchan, A Morley, P |
| author_sort | Small, D |
| collection | OXFORD |
| description | Excessive accumulation of Ca2+ in neurones and glutamate release are involved in neuropathological processes, including ischaemia. We investigated the neuroprotective effects of the Ca2+ channel antagonist, omega-Aga-IVA, in CA1 pyramidal neurones in rat hippocampal slices following an in vitro hypoxic-hypoglycaemic insult. Following this insult, evoked post-synaptic response amplitudes decreased from 3.7 +/- 0.5 mV to 0.6 +/- 0.2 mV and the CA1 neurones appeared dead using a live/dead fluorescence assay with confocal microscopy. Slices treated with 200 nM omega-Aga-IVA had evoked response amplitudes not significantly different from control (3.3 +/- 0.5 mV) and the CA1 neurones appeared viable using the live/dead fluorescence assay. The neuroprotective efficacy of omega-Aga-IVA suggests that omega-Aga-IVA-sensitive Ca2+ channels participate in ischaemic neuronal death and constitute a potential target of therapeutic intervention. |
| first_indexed | 2024-03-07T06:13:41Z |
| format | Journal article |
| id | oxford-uuid:f06434b3-1b6d-403c-be3e-3b453f8c17a3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:13:41Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:f06434b3-1b6d-403c-be3e-3b453f8c17a32022-03-27T11:47:33ZNeuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f06434b3-1b6d-403c-be3e-3b453f8c17a3EnglishSymplectic Elements at Oxford1995Small, DMonette, RMealing, GBuchan, AMorley, PExcessive accumulation of Ca2+ in neurones and glutamate release are involved in neuropathological processes, including ischaemia. We investigated the neuroprotective effects of the Ca2+ channel antagonist, omega-Aga-IVA, in CA1 pyramidal neurones in rat hippocampal slices following an in vitro hypoxic-hypoglycaemic insult. Following this insult, evoked post-synaptic response amplitudes decreased from 3.7 +/- 0.5 mV to 0.6 +/- 0.2 mV and the CA1 neurones appeared dead using a live/dead fluorescence assay with confocal microscopy. Slices treated with 200 nM omega-Aga-IVA had evoked response amplitudes not significantly different from control (3.3 +/- 0.5 mV) and the CA1 neurones appeared viable using the live/dead fluorescence assay. The neuroprotective efficacy of omega-Aga-IVA suggests that omega-Aga-IVA-sensitive Ca2+ channels participate in ischaemic neuronal death and constitute a potential target of therapeutic intervention. |
| spellingShingle | Small, D Monette, R Mealing, G Buchan, A Morley, P Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title | Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title_full | Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title_fullStr | Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title_full_unstemmed | Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title_short | Neuroprotective effects of omega-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. |
| title_sort | neuroprotective effects of omega aga iva against in vitro ischaemia in the rat hippocampal slice |
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