Surfing on a new wave of single-molecule fluorescence methods.
Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are characterized by a substantial increase in complexity of the instrumentation o...
| Main Authors: | , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado: |
2010
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| _version_ | 1826304199153942528 |
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| author | Hohlbein, J Gryte, K Heilemann, M Kapanidis, A |
| author_facet | Hohlbein, J Gryte, K Heilemann, M Kapanidis, A |
| author_sort | Hohlbein, J |
| collection | OXFORD |
| description | Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are characterized by a substantial increase in complexity of the instrumentation or biological samples involved. Specifically, we describe new multi-laser and multi-colour fluorescence spectroscopy and imaging techniques, super-resolution microscopy imaging and the development of instruments that combine fluorescence detection with other single-molecule methods such as force spectroscopy. We also highlight two pivotal developments in basic and applied biosciences: the new information available from detection of single molecules in single biological cells and exciting developments in fluorescence-based single-molecule DNA sequencing. |
| first_indexed | 2024-03-07T06:14:10Z |
| format | Journal article |
| id | oxford-uuid:f08916d9-e45e-42b5-8766-b36ecdbaf72d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:14:10Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:f08916d9-e45e-42b5-8766-b36ecdbaf72d2022-03-27T11:48:42ZSurfing on a new wave of single-molecule fluorescence methods.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f08916d9-e45e-42b5-8766-b36ecdbaf72dEnglishSymplectic Elements at Oxford2010Hohlbein, JGryte, KHeilemann, MKapanidis, ASingle-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are characterized by a substantial increase in complexity of the instrumentation or biological samples involved. Specifically, we describe new multi-laser and multi-colour fluorescence spectroscopy and imaging techniques, super-resolution microscopy imaging and the development of instruments that combine fluorescence detection with other single-molecule methods such as force spectroscopy. We also highlight two pivotal developments in basic and applied biosciences: the new information available from detection of single molecules in single biological cells and exciting developments in fluorescence-based single-molecule DNA sequencing. |
| spellingShingle | Hohlbein, J Gryte, K Heilemann, M Kapanidis, A Surfing on a new wave of single-molecule fluorescence methods. |
| title | Surfing on a new wave of single-molecule fluorescence methods. |
| title_full | Surfing on a new wave of single-molecule fluorescence methods. |
| title_fullStr | Surfing on a new wave of single-molecule fluorescence methods. |
| title_full_unstemmed | Surfing on a new wave of single-molecule fluorescence methods. |
| title_short | Surfing on a new wave of single-molecule fluorescence methods. |
| title_sort | surfing on a new wave of single molecule fluorescence methods |
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