A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells.
Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous method...
| Κύριοι συγγραφείς: | , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
2011
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| _version_ | 1826304288690798592 |
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| author | Charles, E Orloff, M Dustin, L |
| author_facet | Charles, E Orloff, M Dustin, L |
| author_sort | Charles, E |
| collection | OXFORD |
| description | Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous methods to develop a flow cytometry-based method to efficiently clone monoclonal antibodies (mAbs) from humans with expansions of RF-like, immunoglobulin heavy chain variable region (IgVH) 1-69 gene segment-containing B cells. The cloned variable regions are expressed as IgM and produced during culture at concentrations between 5 and 20 μg/ml. Using this system, we show that clonal Igs from patients with HCV-related mixed cryoglobulinemia, when expressed as IgM, have RF activity. We anticipate that this system will be useful for the cloning and expression of mAbs partially encoded by VH1-69 and for determination of the reactivity patterns of polyspecific, low-affinity IgMs of human pathogenic importance. |
| first_indexed | 2024-03-07T06:15:32Z |
| format | Journal article |
| id | oxford-uuid:f0f6b1b3-a37e-44d2-a58b-ee6ab37a3bc8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:15:32Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:f0f6b1b3-a37e-44d2-a58b-ee6ab37a3bc82022-03-27T11:52:06ZA flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f0f6b1b3-a37e-44d2-a58b-ee6ab37a3bc8EnglishSymplectic Elements at Oxford2011Charles, EOrloff, MDustin, LPathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous methods to develop a flow cytometry-based method to efficiently clone monoclonal antibodies (mAbs) from humans with expansions of RF-like, immunoglobulin heavy chain variable region (IgVH) 1-69 gene segment-containing B cells. The cloned variable regions are expressed as IgM and produced during culture at concentrations between 5 and 20 μg/ml. Using this system, we show that clonal Igs from patients with HCV-related mixed cryoglobulinemia, when expressed as IgM, have RF activity. We anticipate that this system will be useful for the cloning and expression of mAbs partially encoded by VH1-69 and for determination of the reactivity patterns of polyspecific, low-affinity IgMs of human pathogenic importance. |
| spellingShingle | Charles, E Orloff, M Dustin, L A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title | A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title_full | A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title_fullStr | A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title_full_unstemmed | A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title_short | A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells. |
| title_sort | flow cytometry based strategy to identify and express igm from vh1 69 clonal peripheral b cells |
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