The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance.
The kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distanc...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1826304369842192384 |
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| author | Pierres, A Benoliel, A Bongrand, P Van Der Merwe, P |
| author_facet | Pierres, A Benoliel, A Bongrand, P Van Der Merwe, P |
| author_sort | Pierres, A |
| collection | OXFORD |
| description | The kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distance from the velocity, thus yielding a relationship between this distance and the binding rate. Numerical values of the binding site densities allowed absolute determination of the rate of association between two individual molecules as a function of the distance between attachment points. In our model, this rate was about 0.03 s-1 at 10 nm separation, and it was inversely proportional to the cube of the distance. |
| first_indexed | 2024-03-07T06:16:45Z |
| format | Journal article |
| id | oxford-uuid:f1543b5d-709f-49d2-ac6a-ba45268699d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:16:45Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:f1543b5d-709f-49d2-ac6a-ba45268699d92022-03-27T11:55:19ZThe dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f1543b5d-709f-49d2-ac6a-ba45268699d9EnglishSymplectic Elements at Oxford1997Pierres, ABenoliel, ABongrand, PVan Der Merwe, PThe kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distance from the velocity, thus yielding a relationship between this distance and the binding rate. Numerical values of the binding site densities allowed absolute determination of the rate of association between two individual molecules as a function of the distance between attachment points. In our model, this rate was about 0.03 s-1 at 10 nm separation, and it was inversely proportional to the cube of the distance. |
| spellingShingle | Pierres, A Benoliel, A Bongrand, P Van Der Merwe, P The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title | The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title_full | The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title_fullStr | The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title_full_unstemmed | The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title_short | The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. |
| title_sort | dependence of the association rate of surface attached adhesion molecules cd2 and cd48 on separation distance |
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