Establishment of the European meningococcal strain collection genome library (EMSC-GL)
Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC i...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
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European Centre for Disease Prevention and Control
2018
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| _version_ | 1797103230643077120 |
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| author | Bratcher, HB Brehony, C Heuberger, S Pieridou-Bagatzouni, D Krizova, P Hoffmann, S Toropainen, M Taha, MK Claus, H Tzanakaki, G Erdosi, T Galajeva, J Van der Ende, A Skoczynska, A Pana, M Vaculikova, A Paragi, M Maiden, MCJ Caugant, D |
| author_facet | Bratcher, HB Brehony, C Heuberger, S Pieridou-Bagatzouni, D Krizova, P Hoffmann, S Toropainen, M Taha, MK Claus, H Tzanakaki, G Erdosi, T Galajeva, J Van der Ende, A Skoczynska, A Pana, M Vaculikova, A Paragi, M Maiden, MCJ Caugant, D |
| author_sort | Bratcher, HB |
| collection | OXFORD |
| description | Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011–June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neisseria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated genotypes in Europe, enabling the monitoring of hyperinvasive lineages, outbreak identification, and supporting vaccine programme implementation. |
| first_indexed | 2024-03-07T06:17:07Z |
| format | Journal article |
| id | oxford-uuid:f1748dc7-e623-41c0-8a9a-144459fb98d7 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:17:07Z |
| publishDate | 2018 |
| publisher | European Centre for Disease Prevention and Control |
| record_format | dspace |
| spelling | oxford-uuid:f1748dc7-e623-41c0-8a9a-144459fb98d72022-03-27T11:56:11ZEstablishment of the European meningococcal strain collection genome library (EMSC-GL)Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f1748dc7-e623-41c0-8a9a-144459fb98d7Symplectic Elements at OxfordEuropean Centre for Disease Prevention and Control2018Bratcher, HBBrehony, CHeuberger, SPieridou-Bagatzouni, DKrizova, PHoffmann, SToropainen, MTaha, MKClaus, HTzanakaki, GErdosi, TGalajeva, JVan der Ende, ASkoczynska, APana, MVaculikova, AParagi, MMaiden, MCJCaugant, DInvasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011–June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neisseria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated genotypes in Europe, enabling the monitoring of hyperinvasive lineages, outbreak identification, and supporting vaccine programme implementation. |
| spellingShingle | Bratcher, HB Brehony, C Heuberger, S Pieridou-Bagatzouni, D Krizova, P Hoffmann, S Toropainen, M Taha, MK Claus, H Tzanakaki, G Erdosi, T Galajeva, J Van der Ende, A Skoczynska, A Pana, M Vaculikova, A Paragi, M Maiden, MCJ Caugant, D Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title | Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title_full | Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title_fullStr | Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title_full_unstemmed | Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title_short | Establishment of the European meningococcal strain collection genome library (EMSC-GL) |
| title_sort | establishment of the european meningococcal strain collection genome library emsc gl |
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