Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics.
The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here,...
| Auteurs principaux: | , , , , , , , , |
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| Format: | Journal article |
| Langue: | English |
| Publié: |
2009
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| _version_ | 1826304421775015936 |
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| author | Lenshof, A Ahmad-Tajudin, A Järås, K Swärd-Nilsson, A Aberg, L Marko-Varga, G Malm, J Lilja, H Laurell, T |
| author_facet | Lenshof, A Ahmad-Tajudin, A Järås, K Swärd-Nilsson, A Aberg, L Marko-Varga, G Malm, J Lilja, H Laurell, T |
| author_sort | Lenshof, A |
| collection | OXFORD |
| description | The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL). |
| first_indexed | 2024-03-07T06:17:33Z |
| format | Journal article |
| id | oxford-uuid:f19c2eab-bc48-4e01-acb3-3a4bbe238aba |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:17:33Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:f19c2eab-bc48-4e01-acb3-3a4bbe238aba2022-03-27T11:57:19ZAcoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f19c2eab-bc48-4e01-acb3-3a4bbe238abaEnglishSymplectic Elements at Oxford2009Lenshof, AAhmad-Tajudin, AJärås, KSwärd-Nilsson, AAberg, LMarko-Varga, GMalm, JLilja, HLaurell, TThe generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL). |
| spellingShingle | Lenshof, A Ahmad-Tajudin, A Järås, K Swärd-Nilsson, A Aberg, L Marko-Varga, G Malm, J Lilja, H Laurell, T Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title_full | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title_fullStr | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title_full_unstemmed | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title_short | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics. |
| title_sort | acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics |
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