Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation
<strong>Objectives</strong> Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Stu...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
BioMed Central
2018
|
| _version_ | 1797103397811257344 |
|---|---|
| author | Alam, MS Kibria, MG Jahan, N Price, RN Ley, B |
| author_facet | Alam, MS Kibria, MG Jahan, N Price, RN Ley, B |
| author_sort | Alam, MS |
| collection | OXFORD |
| description | <strong>Objectives</strong> Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. <strong>Results</strong> The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: − 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0–100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4–100.0) and 97.1% (95% CI 84.7–99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity. |
| first_indexed | 2024-03-07T06:19:36Z |
| format | Journal article |
| id | oxford-uuid:f24797dc-17f7-42f7-aebe-c48e414263e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:19:36Z |
| publishDate | 2018 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:f24797dc-17f7-42f7-aebe-c48e414263e92022-03-27T12:02:24ZSpectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f24797dc-17f7-42f7-aebe-c48e414263e9EnglishSymplectic Elements at OxfordBioMed Central2018Alam, MSKibria, MGJahan, NPrice, RNLey, B<strong>Objectives</strong> Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. <strong>Results</strong> The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: − 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0–100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4–100.0) and 97.1% (95% CI 84.7–99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity. |
| spellingShingle | Alam, MS Kibria, MG Jahan, N Price, RN Ley, B Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title | Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title_full | Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title_fullStr | Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title_full_unstemmed | Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title_short | Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation |
| title_sort | spectrophotometry assays to determine g6pd activity from trinity biotech and pointe scientific g6pd show good correlation |
| work_keys_str_mv | AT alamms spectrophotometryassaystodetermineg6pdactivityfromtrinitybiotechandpointescientificg6pdshowgoodcorrelation AT kibriamg spectrophotometryassaystodetermineg6pdactivityfromtrinitybiotechandpointescientificg6pdshowgoodcorrelation AT jahann spectrophotometryassaystodetermineg6pdactivityfromtrinitybiotechandpointescientificg6pdshowgoodcorrelation AT pricern spectrophotometryassaystodetermineg6pdactivityfromtrinitybiotechandpointescientificg6pdshowgoodcorrelation AT leyb spectrophotometryassaystodetermineg6pdactivityfromtrinitybiotechandpointescientificg6pdshowgoodcorrelation |