Far-field fluorescence microscopy at the macromolecular scale

Far-field fluorescence microscopy at the macromolecular scale

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Since its discovery by Abbe in 1873, the microscopy diffraction barrier has received a lot of attention. In this lecture, we discuss the principle of fundamentally breaking the diffraction barrier through reversible saturable optical (fluorescence) transitions (RESOLFT). © 2005 Optical Society of Am...

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Bibliografiske detaljer
Main Authors:	Hell, S, Willig, K, Hofmann, M, Eggeling, C, Westphal, V
Format:	Conference item
Udgivet:	Optical Society of America 2006

Lignende værker

Far-field fluorescence microscopy at the macromolecular scale
af: Hell, S, et al.
Udgivet: (2006)

Macromolecular-scale resolution in biological fluorescence microscopy.
af: Donnert, G, et al.
Udgivet: (2006)

Two-color far-field fluorescence nanoscopy.
af: Donnert, G, et al.
Udgivet: (2007)

Studying nanoscale membrane dynamics by sub-diffraction far-field fluorescence STED microscopy
af: Eggeling, C, et al.
Udgivet: (2009)

Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching.
af: Schwentker, M, et al.
Udgivet: (2007)

Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
af: Hofmann, M, et al.
Udgivet: (2005)

STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue.
af: Honigmann, A, et al.
Udgivet: (2013)

Breaking the diffraction barrier in fluorescence microscopy by optical shelving.
af: Bretschneider, S, et al.
Udgivet: (2007)

Breaking the diffraction barrier in fluorescence microscopy by optical shelving
af: Bretschneider, S, et al.
Udgivet: (2007)

Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy.
af: Stiel, A, et al.
Udgivet: (2008)

Major signal increase in fluorescence microscopy through dark-state relaxation.
af: Donnert, G, et al.
Udgivet: (2007)

Two-color far-field fluorescence nanoscopy based on photoswitchable emitters
af: Bock, H, et al.
Udgivet: (2007)

Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species.
af: Bossi, M, et al.
Udgivet: (2008)

Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy.
af: Kolmakov, K, et al.
Udgivet: (2010)

Far-field autofluorescence nanoscopy.
af: Bierwagen, J, et al.
Udgivet: (2010)

Far-field electrostatic signatures of macromolecular 3D conformation
af: Kloes, G, et al.
Udgivet: (2022)

Far-field optical nanoscopy of diamond color centers
af: Rittweger, E, et al.
Udgivet: (2009)

Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light.
af: Han, K, et al.
Udgivet: (2009)

Far-field optical nanoscopy of diamond color centers
af: Rittweger, E, et al.
Udgivet: (2009)

STED microscopy made simple
af: Schoenle, A, et al.
Udgivet: (2009)

Analytical description of STED microscopy performance.
af: Leutenegger, M, et al.
Udgivet: (2010)

STED microscopy of living cells - New frontiers in membrane and neurobiology
af: Eggeling, C, et al.
Udgivet: (2013)

STED microscopy of living cells--new frontiers in membrane and neurobiology.
af: Eggeling, C, et al.
Udgivet: (2013)

Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching
af: Geisler, C, et al.
Udgivet: (2007)

Triplet-relaxation microscopy with bunched pulsed excitation.
af: Donnert, G, et al.
Udgivet: (2009)

Fluorescence Microscopy of the HIV-1 Envelope
af: Pablo Carravilla, et al.
Udgivet: (2020-03-01)

Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS).
af: Leutenegger, M, et al.
Udgivet: (2012)

Super-resolution fluorescence microscopy studies of human immunodeficiency virus
af: Jakub Chojnacki, et al.
Udgivet: (2018-06-01)

Metastable dark States enable ground state depletion microscopy of nitrogen vacancy centers in diamond with diffraction-unlimited resolution.
af: Han, K, et al.
Udgivet: (2010)

Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
af: Kastrup, L, et al.
Udgivet: (2005)

Fluorescence fluctuation spectroscopy in subdiffraction focal volumes.
af: Kastrup, L, et al.
Udgivet: (2005)

Far-field super-resolution chemical microscopy
af: Mingwei Tang, et al.
Udgivet: (2023-06-01)

STED microscopy reveals crystal colour centres with nanometric resolution
af: Rittweger, E, et al.
Udgivet: (2009)

High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording
af: Sahl, S, et al.
Udgivet: (2014)

High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording.
af: Sahl, S, et al.
Udgivet: (2014)

Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes
af: Slavica Jonić
Udgivet: (2016-01-01)

Molecular mechanism of the reversible photoswitch in the fluorescent protein asFP595
af: Andresen, M, et al.
Udgivet: (2006)

Nanoscopy of living brain slices with low light levels.
af: Testa, I, et al.
Udgivet: (2012)

Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy.
af: Ringemann, C, et al.
Udgivet: (2008)

Far-field and near-field photon emission microscopy for semiconductor devices
af: Ding, Yuzhou
Udgivet: (2012)