WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription
Nuclear gene transcription is coordinated with transcript release from the chromatin template and messenger RNA export to the cytoplasm. We describe the role of nuclear localized kinase WNK1 in the mammalian mRNA export pathway, even though previously established as a critical regulator of ion homeo...
| Main Authors: | , , , |
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| Format: | Journal article |
| Published: |
Cold Spring Harbor Laboratory Press
2017
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| Summary: | Nuclear gene transcription is coordinated with transcript release from the chromatin template and messenger RNA export to the cytoplasm. We describe the role of nuclear localized kinase WNK1 in the mammalian mRNA export pathway, even though previously established as a critical regulator of ion homeostasis in the cytoplasm. Our data reveal that WNK1 phosphorylates the termination factor PCF11 on its RNA polymerase II (Pol II) CTD interacting domain (CID). Furthermore, phosphorylation of PCF11 CID weakens its interaction with Pol II. We predict that WNK1 and the associated phosphorylation of the PCF11 CID acts to promote transcript release from chromatin-associated Pol II. This in turn facilitates mRNA export to the cytoplasm. |
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