Enhanced photocurrent in engineered bacteriorhodopsin monolayer.
The integration of the transmembrane protein bacteriorhodopsin (BR) with man-made electrode surfaces has attracted a great deal of interest for some two decades or more and holds significant promise from the perspective of derived photoresponse or energy capture interfaces. Here we demonstrate that...
| Autores principales: | , , , , |
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| Formato: | Journal article |
| Lenguaje: | English |
| Publicado: |
2012
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| _version_ | 1826304690510364672 |
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| author | Patil, A Premaruban, T Berthoumieu, O Watts, A Davis, J |
| author_facet | Patil, A Premaruban, T Berthoumieu, O Watts, A Davis, J |
| author_sort | Patil, A |
| collection | OXFORD |
| description | The integration of the transmembrane protein bacteriorhodopsin (BR) with man-made electrode surfaces has attracted a great deal of interest for some two decades or more and holds significant promise from the perspective of derived photoresponse or energy capture interfaces. Here we demonstrate that a novel and strategically engineered cysteine site (M163C) can be used to intimately and effectively couple delipidated BR to supporting metallic electrode surfaces. By virtue of the combined effects of the greater surface molecular density afforded by delipidation, and the vicinity of the electrostatic changes associated with proton pumping to the transducing metallic continuum, the resulting films generate a considerably greater photocurrent density on wavelength-selective illumination than previously achievable with monolayers of BR. Given the uniquely photoresponsive, wavelength-selective, and photostable characteristics of this protein, the work has implications for utilization in solar energy capture and photodetector devices. |
| first_indexed | 2024-03-07T06:21:38Z |
| format | Journal article |
| id | oxford-uuid:f2e7b66c-d639-47f3-b6ee-c04058ebe04d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:21:38Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:f2e7b66c-d639-47f3-b6ee-c04058ebe04d2022-03-27T12:07:46ZEnhanced photocurrent in engineered bacteriorhodopsin monolayer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f2e7b66c-d639-47f3-b6ee-c04058ebe04dEnglishSymplectic Elements at Oxford2012Patil, APremaruban, TBerthoumieu, OWatts, ADavis, JThe integration of the transmembrane protein bacteriorhodopsin (BR) with man-made electrode surfaces has attracted a great deal of interest for some two decades or more and holds significant promise from the perspective of derived photoresponse or energy capture interfaces. Here we demonstrate that a novel and strategically engineered cysteine site (M163C) can be used to intimately and effectively couple delipidated BR to supporting metallic electrode surfaces. By virtue of the combined effects of the greater surface molecular density afforded by delipidation, and the vicinity of the electrostatic changes associated with proton pumping to the transducing metallic continuum, the resulting films generate a considerably greater photocurrent density on wavelength-selective illumination than previously achievable with monolayers of BR. Given the uniquely photoresponsive, wavelength-selective, and photostable characteristics of this protein, the work has implications for utilization in solar energy capture and photodetector devices. |
| spellingShingle | Patil, A Premaruban, T Berthoumieu, O Watts, A Davis, J Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title | Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title_full | Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title_fullStr | Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title_full_unstemmed | Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title_short | Enhanced photocurrent in engineered bacteriorhodopsin monolayer. |
| title_sort | enhanced photocurrent in engineered bacteriorhodopsin monolayer |
| work_keys_str_mv | AT patila enhancedphotocurrentinengineeredbacteriorhodopsinmonolayer AT premarubant enhancedphotocurrentinengineeredbacteriorhodopsinmonolayer AT berthoumieuo enhancedphotocurrentinengineeredbacteriorhodopsinmonolayer AT wattsa enhancedphotocurrentinengineeredbacteriorhodopsinmonolayer AT davisj enhancedphotocurrentinengineeredbacteriorhodopsinmonolayer |