Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound
Ultrasound-triggered drug-loaded microbubbles have great potential for drug delivery due to their ability to locally release drugs and simultaneously enhance their delivery into the target tissue. We have recently shown that upon applying ultrasound, nanoparticle-loaded microbubbles can deposit nano...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2019
|
| _version_ | 1797103562140942336 |
|---|---|
| author | Roovers, S Deprez, J Priwitaningrum, D Lajoinie, G Rivron, N Declercq, H De Wever, O Stride, E Le Gac, S Versluis, M Prakash, J De Smedt, SC Lentacker, I |
| author_facet | Roovers, S Deprez, J Priwitaningrum, D Lajoinie, G Rivron, N Declercq, H De Wever, O Stride, E Le Gac, S Versluis, M Prakash, J De Smedt, SC Lentacker, I |
| author_sort | Roovers, S |
| collection | OXFORD |
| description | Ultrasound-triggered drug-loaded microbubbles have great potential for drug delivery due to their ability to locally release drugs and simultaneously enhance their delivery into the target tissue. We have recently shown that upon applying ultrasound, nanoparticle-loaded microbubbles can deposit nanoparticles onto cells grown in 2D monolayers, through a process that we termed "sonoprinting". However, the rigid surfaces on which cell monolayers are typically growing might be a source of acoustic reflections and aspherical microbubble oscillations, which can influence microbubble-cell interactions. In the present study, we aim to reveal whether sonoprinting can also occur in more complex and physiologically relevant tissues, by using free-floating 3D tumor spheroids as a tissue model. We show that both monospheroids (consisting of tumor cells alone) and cospheroids (consisting of tumor cells and fibroblasts, which produce an extracellular matrix) can be sonoprinted. Using doxorubicin-liposome-loaded microbubbles, we show that sonoprinting allows to deposit large amounts of doxorubicin-containing liposomes to the outer cell layers of the spheroids, followed by doxorubicin release into the deeper layers of the spheroids, resulting in a significant reduction in cell viability. Sonoprinting may become an attractive approach to deposit drug patches at the surface of tissues, thereby promoting the delivery of drugs into target tissues. |
| first_indexed | 2024-03-07T06:21:51Z |
| format | Journal article |
| id | oxford-uuid:f2f8995d-9d50-4de7-8246-2766d08ba8e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:21:51Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:f2f8995d-9d50-4de7-8246-2766d08ba8e92022-03-27T12:08:17ZSonoprinting liposomes on tumor spheroids by microbubbles and ultrasoundJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f2f8995d-9d50-4de7-8246-2766d08ba8e9EnglishSymplectic ElementsElsevier2019Roovers, SDeprez, JPriwitaningrum, DLajoinie, GRivron, NDeclercq, HDe Wever, OStride, ELe Gac, SVersluis, MPrakash, JDe Smedt, SCLentacker, IUltrasound-triggered drug-loaded microbubbles have great potential for drug delivery due to their ability to locally release drugs and simultaneously enhance their delivery into the target tissue. We have recently shown that upon applying ultrasound, nanoparticle-loaded microbubbles can deposit nanoparticles onto cells grown in 2D monolayers, through a process that we termed "sonoprinting". However, the rigid surfaces on which cell monolayers are typically growing might be a source of acoustic reflections and aspherical microbubble oscillations, which can influence microbubble-cell interactions. In the present study, we aim to reveal whether sonoprinting can also occur in more complex and physiologically relevant tissues, by using free-floating 3D tumor spheroids as a tissue model. We show that both monospheroids (consisting of tumor cells alone) and cospheroids (consisting of tumor cells and fibroblasts, which produce an extracellular matrix) can be sonoprinted. Using doxorubicin-liposome-loaded microbubbles, we show that sonoprinting allows to deposit large amounts of doxorubicin-containing liposomes to the outer cell layers of the spheroids, followed by doxorubicin release into the deeper layers of the spheroids, resulting in a significant reduction in cell viability. Sonoprinting may become an attractive approach to deposit drug patches at the surface of tissues, thereby promoting the delivery of drugs into target tissues. |
| spellingShingle | Roovers, S Deprez, J Priwitaningrum, D Lajoinie, G Rivron, N Declercq, H De Wever, O Stride, E Le Gac, S Versluis, M Prakash, J De Smedt, SC Lentacker, I Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title | Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title_full | Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title_fullStr | Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title_full_unstemmed | Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title_short | Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| title_sort | sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound |
| work_keys_str_mv | AT rooverss sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT deprezj sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT priwitaningrumd sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT lajoinieg sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT rivronn sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT declercqh sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT dewevero sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT stridee sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT legacs sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT versluism sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT prakashj sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT desmedtsc sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound AT lentackeri sonoprintingliposomesontumorspheroidsbymicrobubblesandultrasound |