Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS.

Proteins often exist as ensembles of interconverting states in solution which are often difficult to quantify. In the present manuscript we show that the combination of MS under nondenaturing conditions and AUC-SV (analytical ultracentrifugation sedimentation velocity) unambiguously clarifies a dist...

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Main Authors: Noda, M, Uchiyama, S, McKay, A, Morimoto, A, Misawa, S, Yoshida, A, Shimahara, H, Takinowaki, H, Nakamura, S, Kobayashi, Y, Matsunaga, S, Ohkubo, T, Robinson, C, Fukui, K
Format: Journal article
Jezik:English
Izdano: 2011