Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
The properties of human lymphocyte fractions isolated either by sheep red cell(E) rosetting or by fluorescence-activated cell sorting after staining with UCHT1 monoclonal anti-T cell antibody have been compared. Two populations of E+ cells with very different phenotype and function have been identif...
| Hlavní autoři: | , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
1981
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| _version_ | 1826304774087114752 |
|---|---|
| author | Beverley, P Callard, R |
| author_facet | Beverley, P Callard, R |
| author_sort | Beverley, P |
| collection | OXFORD |
| description | The properties of human lymphocyte fractions isolated either by sheep red cell(E) rosetting or by fluorescence-activated cell sorting after staining with UCHT1 monoclonal anti-T cell antibody have been compared. Two populations of E+ cells with very different phenotype and function have been identified. E+/UCHT1+ cells respond well to the T cell mitogens phytohemagglutinin and concanavalin A and provide help for an in vitro specific antibody response. They can also suppress the antibody response of allegeneic peripheral blood mononuclear cells. In contrast, the E+/UCHT1- population, which has no other markers characteristic of T cells, fails to respond to mitogens or to provide help or suppression for an antibody response. These cells, however, are highly active natural killers. They possess Fc gamma receptors and have a characteristic staining pattern of nonspecific esterase enzyme activity. It is concluded that not all cells capable of forming E rosettes are thymus-processed cells and that this heterogeneity can be revealed by staining with the monoclonal anti-T cell reagent UCHT1. |
| first_indexed | 2024-03-07T06:22:52Z |
| format | Journal article |
| id | oxford-uuid:f3435c67-dbc7-4aac-83a4-533eed3853e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:22:52Z |
| publishDate | 1981 |
| record_format | dspace |
| spelling | oxford-uuid:f3435c67-dbc7-4aac-83a4-533eed3853e92022-03-27T12:10:49ZDistinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f3435c67-dbc7-4aac-83a4-533eed3853e9EnglishSymplectic Elements at Oxford1981Beverley, PCallard, RThe properties of human lymphocyte fractions isolated either by sheep red cell(E) rosetting or by fluorescence-activated cell sorting after staining with UCHT1 monoclonal anti-T cell antibody have been compared. Two populations of E+ cells with very different phenotype and function have been identified. E+/UCHT1+ cells respond well to the T cell mitogens phytohemagglutinin and concanavalin A and provide help for an in vitro specific antibody response. They can also suppress the antibody response of allegeneic peripheral blood mononuclear cells. In contrast, the E+/UCHT1- population, which has no other markers characteristic of T cells, fails to respond to mitogens or to provide help or suppression for an antibody response. These cells, however, are highly active natural killers. They possess Fc gamma receptors and have a characteristic staining pattern of nonspecific esterase enzyme activity. It is concluded that not all cells capable of forming E rosettes are thymus-processed cells and that this heterogeneity can be revealed by staining with the monoclonal anti-T cell reagent UCHT1. |
| spellingShingle | Beverley, P Callard, R Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title | Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title_full | Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title_fullStr | Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title_full_unstemmed | Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title_short | Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
| title_sort | distinctive functional characteristics of human t lymphocytes defined by e rosetting or a monoclonal anti t cell antibody |
| work_keys_str_mv | AT beverleyp distinctivefunctionalcharacteristicsofhumantlymphocytesdefinedbyerosettingoramonoclonalantitcellantibody AT callardr distinctivefunctionalcharacteristicsofhumantlymphocytesdefinedbyerosettingoramonoclonalantitcellantibody |