Applications of RNA interference high-throughput screening technology in cancer biology and virology.

RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables resea...

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Detalles Bibliográficos
Autores principales: Gao, S, Yang, C, Jiang, S, Xu, X, Lu, X, He, Y, Cheung, A, Wang, H
Formato: Journal article
Lenguaje:English
Publicado: 2014
Descripción
Sumario:RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.