Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'.
Previous data suggest that somatostatin is present and released from the hypothalamus in several molecular forms, basally and after K+ or electrical stimulation. In order to evaluate the proportions of somatostatin-14 (S14) and somatostatin-28 (S28) released during a stimulus which may be more close...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1984
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| _version_ | 1826304909276872704 |
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| author | Lengyel, A Nieuwenhuyzen Kruseman, A Grossman, A Rees, L Besser, G |
| author_facet | Lengyel, A Nieuwenhuyzen Kruseman, A Grossman, A Rees, L Besser, G |
| author_sort | Lengyel, A |
| collection | OXFORD |
| description | Previous data suggest that somatostatin is present and released from the hypothalamus in several molecular forms, basally and after K+ or electrical stimulation. In order to evaluate the proportions of somatostatin-14 (S14) and somatostatin-28 (S28) released during a stimulus which may be more closely related to the control of growth hormone secretion 'in vivo', we studied the molecular forms of somatostatin released from hypothalamic fragments ' in vitro', during incubations with different glucose concentrations (1.35 and 22mM), which we have previously shown to be inversely related to somatostatin release. Sephadex G-50 chromatography demonstrated that both forms are released in the same proportions (S14: 70%; S28: 30%) during incubation with different glucose concentrations; there is a parallel increase in both forms when low glucose is used. Although on a molar basis less S28 is released than S14, the higher potency, longer duration of action and higher affinity for pituitary receptors of S28 suggests that it may be of major physiological importance. |
| first_indexed | 2024-03-07T06:24:51Z |
| format | Journal article |
| id | oxford-uuid:f3ef3102-f6fb-4dde-9a98-994ea1bd1b0c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:24:51Z |
| publishDate | 1984 |
| record_format | dspace |
| spelling | oxford-uuid:f3ef3102-f6fb-4dde-9a98-994ea1bd1b0c2022-03-27T12:15:50ZGlucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f3ef3102-f6fb-4dde-9a98-994ea1bd1b0cEnglishSymplectic Elements at Oxford1984Lengyel, ANieuwenhuyzen Kruseman, AGrossman, ARees, LBesser, GPrevious data suggest that somatostatin is present and released from the hypothalamus in several molecular forms, basally and after K+ or electrical stimulation. In order to evaluate the proportions of somatostatin-14 (S14) and somatostatin-28 (S28) released during a stimulus which may be more closely related to the control of growth hormone secretion 'in vivo', we studied the molecular forms of somatostatin released from hypothalamic fragments ' in vitro', during incubations with different glucose concentrations (1.35 and 22mM), which we have previously shown to be inversely related to somatostatin release. Sephadex G-50 chromatography demonstrated that both forms are released in the same proportions (S14: 70%; S28: 30%) during incubation with different glucose concentrations; there is a parallel increase in both forms when low glucose is used. Although on a molar basis less S28 is released than S14, the higher potency, longer duration of action and higher affinity for pituitary receptors of S28 suggests that it may be of major physiological importance. |
| spellingShingle | Lengyel, A Nieuwenhuyzen Kruseman, A Grossman, A Rees, L Besser, G Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title | Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title_full | Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title_fullStr | Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title_full_unstemmed | Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title_short | Glucose-induced changes in somatostatin-14 and somatostatin-28 released from rat hypothalamic fragments 'in vitro'. |
| title_sort | glucose induced changes in somatostatin 14 and somatostatin 28 released from rat hypothalamic fragments in vitro |
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