Structure of a H3N2 influenza virus nucleoprotein
Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structur...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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International Union of Crystallography
2021
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| _version_ | 1826304986514980864 |
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| author | Knight, M Fan, H Bauer, D Grimes, J Fodor, E Keown, J |
| author_facet | Knight, M Fan, H Bauer, D Grimes, J Fodor, E Keown, J |
| author_sort | Knight, M |
| collection | OXFORD |
| description | Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza.
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| first_indexed | 2024-03-07T06:26:02Z |
| format | Journal article |
| id | oxford-uuid:f4524153-d456-4807-921e-355e6afe912c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:26:02Z |
| publishDate | 2021 |
| publisher | International Union of Crystallography |
| record_format | dspace |
| spelling | oxford-uuid:f4524153-d456-4807-921e-355e6afe912c2022-03-27T12:19:01ZStructure of a H3N2 influenza virus nucleoproteinJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f4524153-d456-4807-921e-355e6afe912cEnglishSymplectic ElementsInternational Union of Crystallography2021Knight, MFan, HBauer, DGrimes, JFodor, EKeown, JInfluenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza. |
| spellingShingle | Knight, M Fan, H Bauer, D Grimes, J Fodor, E Keown, J Structure of a H3N2 influenza virus nucleoprotein |
| title | Structure of a H3N2 influenza virus nucleoprotein |
| title_full | Structure of a H3N2 influenza virus nucleoprotein |
| title_fullStr | Structure of a H3N2 influenza virus nucleoprotein |
| title_full_unstemmed | Structure of a H3N2 influenza virus nucleoprotein |
| title_short | Structure of a H3N2 influenza virus nucleoprotein |
| title_sort | structure of a h3n2 influenza virus nucleoprotein |
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