Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance
Mitochondrial genomes represent a valuable source of data for evolutionary research, but studies of their short-term evolution have typically been limited to invertebrates, humans and laboratory organisms. Here we present a detailed study of 12 mitochondrial genomes that span a total of 385 transmis...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
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Royal Society, The
2015
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| _version_ | 1797103872936771584 |
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| author | Larson, G Alexander, M Ho, S Molak, M Barnett, R Carlborg, O Dorshorst, B Honaker, C Besnier, F Wahlberg, P Dobney, K Siegel, P Andersson, L |
| author_facet | Larson, G Alexander, M Ho, S Molak, M Barnett, R Carlborg, O Dorshorst, B Honaker, C Besnier, F Wahlberg, P Dobney, K Siegel, P Andersson, L |
| author_sort | Larson, G |
| collection | OXFORD |
| description | Mitochondrial genomes represent a valuable source of data for evolutionary research, but studies of their short-term evolution have typically been limited to invertebrates, humans and laboratory organisms. Here we present a detailed study of 12 mitochondrial genomes that span a total of 385 transmissions in a well-documented 50-generation pedigree in which two lineages of chickens were selected for low and high juvenile body weight. These data allowed us to test the hypothesis of time-dependent evolutionary rates and the assumption of strict maternal mitochondrial transmission, and to investigate the role of mitochondrial mutations in determining phenotype. The identification of a non-synonymous mutation inND4Land a synonymous mutation inCYTB, both novel mutations in Gallus, allowed us to estimate a molecular rate of 3.13x10^-7 mutations/site/year (95% confidence interval 3.75x10^-8–1.12^10^-6). This is substantially higher than avian rate estimates based upon fossil calibrations. Ascertaining which of the two novel mutations was present in an additional 49 individuals also revealed an instance of paternal inheritance of mtDNA. Lastly, an association analysis demonstrated that neither of the point mutations was strongly associated with the phenotypic differences between the two selection lines. Together, these observations reveal the highly dynamic nature of mitochondrial evolution over short time periods. |
| first_indexed | 2024-03-07T06:26:11Z |
| format | Journal article |
| id | oxford-uuid:f460a6ab-6d55-42a5-b62d-ab29a8fd4448 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:26:11Z |
| publishDate | 2015 |
| publisher | Royal Society, The |
| record_format | dspace |
| spelling | oxford-uuid:f460a6ab-6d55-42a5-b62d-ab29a8fd44482022-03-27T12:19:21ZMitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritanceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f460a6ab-6d55-42a5-b62d-ab29a8fd4448Symplectic Elements at OxfordRoyal Society, The2015Larson, GAlexander, MHo, SMolak, MBarnett, RCarlborg, ODorshorst, BHonaker, CBesnier, FWahlberg, PDobney, KSiegel, PAndersson, LMitochondrial genomes represent a valuable source of data for evolutionary research, but studies of their short-term evolution have typically been limited to invertebrates, humans and laboratory organisms. Here we present a detailed study of 12 mitochondrial genomes that span a total of 385 transmissions in a well-documented 50-generation pedigree in which two lineages of chickens were selected for low and high juvenile body weight. These data allowed us to test the hypothesis of time-dependent evolutionary rates and the assumption of strict maternal mitochondrial transmission, and to investigate the role of mitochondrial mutations in determining phenotype. The identification of a non-synonymous mutation inND4Land a synonymous mutation inCYTB, both novel mutations in Gallus, allowed us to estimate a molecular rate of 3.13x10^-7 mutations/site/year (95% confidence interval 3.75x10^-8–1.12^10^-6). This is substantially higher than avian rate estimates based upon fossil calibrations. Ascertaining which of the two novel mutations was present in an additional 49 individuals also revealed an instance of paternal inheritance of mtDNA. Lastly, an association analysis demonstrated that neither of the point mutations was strongly associated with the phenotypic differences between the two selection lines. Together, these observations reveal the highly dynamic nature of mitochondrial evolution over short time periods. |
| spellingShingle | Larson, G Alexander, M Ho, S Molak, M Barnett, R Carlborg, O Dorshorst, B Honaker, C Besnier, F Wahlberg, P Dobney, K Siegel, P Andersson, L Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title | Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title_full | Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title_fullStr | Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title_full_unstemmed | Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title_short | Mitogenomic analysis of a 50-generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtDNA inheritance |
| title_sort | mitogenomic analysis of a 50 generation chicken pedigree reveals a rapid rate of mitochondrial evolution and evidence for paternal mtdna inheritance |
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