DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion
Neural crest cells are both highly migratory and significant to vertebrate organogenesis. However, the signals that regulate neural crest cell migration remain unclear. Here, we test the function of DAN, a BMP antagonist we detected by analysis of chick cranial mesoderm. Our analysis shows that, pri...
| Hauptverfasser: | , , , , , , , , , , |
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| Format: | Journal article |
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Rockefeller University Press
2017
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| _version_ | 1826305059243163648 |
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| author | McLennan, R Bailey, C Schumacher, LJ Teddy, J Morrison, J Kasemeier-Kulesa, J Wolfe, L Gogol, M Baker, R Maini, PK Kulesa, P |
| author_facet | McLennan, R Bailey, C Schumacher, LJ Teddy, J Morrison, J Kasemeier-Kulesa, J Wolfe, L Gogol, M Baker, R Maini, PK Kulesa, P |
| author_sort | McLennan, R |
| collection | OXFORD |
| description | Neural crest cells are both highly migratory and significant to vertebrate organogenesis. However, the signals that regulate neural crest cell migration remain unclear. Here, we test the function of DAN, a BMP antagonist we detected by analysis of chick cranial mesoderm. Our analysis shows that, prior to neural crest cell exit from the hindbrain, DAN is expressed in the mesoderm, then it becomes absent along cell migratory pathways. Cranial neural crest and metastatic melanoma cells avoid DAN protein stripes in vitro. Addition of DAN reduces the speed of migrating cells, in vivo and in vitro respectively. In vivo loss-of-function of DAN results in enhanced neural crest cell migration by increasing speed and directionality. Computer model simulations support the hypothesis that DAN restrains cell migration by regulating cell speed. Taken together, our results identify DAN as a novel factor that inhibits uncontrolled neural crest and metastatic melanoma invasion and promotes collective migration in a manner consistent with inhibition of BMP signaling. |
| first_indexed | 2024-03-07T06:27:06Z |
| format | Journal article |
| id | oxford-uuid:f4aec651-89db-4ea9-b93c-0adbe5ffb6ee |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:27:06Z |
| publishDate | 2017 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:f4aec651-89db-4ea9-b93c-0adbe5ffb6ee2022-03-27T12:21:39ZDAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f4aec651-89db-4ea9-b93c-0adbe5ffb6eeSymplectic Elements at OxfordRockefeller University Press2017McLennan, RBailey, CSchumacher, LJTeddy, JMorrison, JKasemeier-Kulesa, JWolfe, LGogol, MBaker, RMaini, PKKulesa, PNeural crest cells are both highly migratory and significant to vertebrate organogenesis. However, the signals that regulate neural crest cell migration remain unclear. Here, we test the function of DAN, a BMP antagonist we detected by analysis of chick cranial mesoderm. Our analysis shows that, prior to neural crest cell exit from the hindbrain, DAN is expressed in the mesoderm, then it becomes absent along cell migratory pathways. Cranial neural crest and metastatic melanoma cells avoid DAN protein stripes in vitro. Addition of DAN reduces the speed of migrating cells, in vivo and in vitro respectively. In vivo loss-of-function of DAN results in enhanced neural crest cell migration by increasing speed and directionality. Computer model simulations support the hypothesis that DAN restrains cell migration by regulating cell speed. Taken together, our results identify DAN as a novel factor that inhibits uncontrolled neural crest and metastatic melanoma invasion and promotes collective migration in a manner consistent with inhibition of BMP signaling. |
| spellingShingle | McLennan, R Bailey, C Schumacher, LJ Teddy, J Morrison, J Kasemeier-Kulesa, J Wolfe, L Gogol, M Baker, R Maini, PK Kulesa, P DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title | DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title_full | DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title_fullStr | DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title_full_unstemmed | DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title_short | DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion |
| title_sort | dan nbl1 promotes collective neural crest migration by restraining uncontrolled invasion |
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