A molecular marker of artemisinin-resistant Plasmodium falciparum malaria

Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasi...

Fuld beskrivelse

Bibliografiske detaljer
Main Authors: Ariey, F, Witkowski, B, Amaratunga, C, Beghain, J, Langlois, A, Khim, N, Kim, S, Duru, V, Bouchier, C, Ma, L, Lim, P, Leang, R, Duong, S, Sreng, S, Suon, S, Chuor, C, Bout, D, Ménard, S, Rogers, W, Genton, B, Fandeur, T, Miotto, O, Ringwald, P, Le Bras, J, Berry, A
Format: Journal article
Sprog:English
Udgivet: 2014
Beskrivelse
Summary:Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7-1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread. © 2014 Macmillan Publishers Limited.