Asymmetric droplet interface bilayers.
In cell membranes, the lipid compositions of the inner and outer leaflets differ. Therefore, a robust model system that enables single-channel electrical recording with asymmetric bilayers would be very useful. We and others recently developed the droplet interface bilayer (DIB), which is formed by...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826305117153918976 |
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| author | Hwang, W Chen, M Cronin, B Holden, M Bayley, H |
| author_facet | Hwang, W Chen, M Cronin, B Holden, M Bayley, H |
| author_sort | Hwang, W |
| collection | OXFORD |
| description | In cell membranes, the lipid compositions of the inner and outer leaflets differ. Therefore, a robust model system that enables single-channel electrical recording with asymmetric bilayers would be very useful. We and others recently developed the droplet interface bilayer (DIB), which is formed by connecting lipid monolayer-encased aqueous droplets submerged in an oil-lipid mixture. Here, we incorporate lipid vesicles of different compositions into aqueous droplets and immerse them in an oil bath to form asymmetric DIBs (a-DIBs). Both alpha-helical and beta-barrel membrane proteins insert readily into a-DIBs, and their activity can be measured by single-channel electrical recording. We show that the gating behavior of outer membrane protein G (OmpG) from Escherichia coli differs depending on the side of insertion in an asymmetric DIB with a positively charged leaflet opposing a negatively charged leaflet. The a-DIB system provides a general platform for studying the effects of bilayer leaflet composition on the behavior of ion channels and pores. |
| first_indexed | 2024-03-07T06:28:00Z |
| format | Journal article |
| id | oxford-uuid:f4fbc07e-5974-4d68-8dfb-0161fc543561 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:28:00Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:f4fbc07e-5974-4d68-8dfb-0161fc5435612022-03-27T12:23:54ZAsymmetric droplet interface bilayers.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f4fbc07e-5974-4d68-8dfb-0161fc543561EnglishSymplectic Elements at Oxford2008Hwang, WChen, MCronin, BHolden, MBayley, HIn cell membranes, the lipid compositions of the inner and outer leaflets differ. Therefore, a robust model system that enables single-channel electrical recording with asymmetric bilayers would be very useful. We and others recently developed the droplet interface bilayer (DIB), which is formed by connecting lipid monolayer-encased aqueous droplets submerged in an oil-lipid mixture. Here, we incorporate lipid vesicles of different compositions into aqueous droplets and immerse them in an oil bath to form asymmetric DIBs (a-DIBs). Both alpha-helical and beta-barrel membrane proteins insert readily into a-DIBs, and their activity can be measured by single-channel electrical recording. We show that the gating behavior of outer membrane protein G (OmpG) from Escherichia coli differs depending on the side of insertion in an asymmetric DIB with a positively charged leaflet opposing a negatively charged leaflet. The a-DIB system provides a general platform for studying the effects of bilayer leaflet composition on the behavior of ion channels and pores. |
| spellingShingle | Hwang, W Chen, M Cronin, B Holden, M Bayley, H Asymmetric droplet interface bilayers. |
| title | Asymmetric droplet interface bilayers. |
| title_full | Asymmetric droplet interface bilayers. |
| title_fullStr | Asymmetric droplet interface bilayers. |
| title_full_unstemmed | Asymmetric droplet interface bilayers. |
| title_short | Asymmetric droplet interface bilayers. |
| title_sort | asymmetric droplet interface bilayers |
| work_keys_str_mv | AT hwangw asymmetricdropletinterfacebilayers AT chenm asymmetricdropletinterfacebilayers AT croninb asymmetricdropletinterfacebilayers AT holdenm asymmetricdropletinterfacebilayers AT bayleyh asymmetricdropletinterfacebilayers |