Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.
This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles.Boar sperm were exposed in vitro to C105Y-functionalized MSNPs...
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| Format: | Journal article |
| Sprache: | English |
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Future Medicine
2015
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| _version_ | 1826305129349906432 |
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| author | Barkalina, N Jones, C Townley, H Coward, K |
| author_facet | Barkalina, N Jones, C Townley, H Coward, K |
| author_sort | Barkalina, N |
| collection | OXFORD |
| description | This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles.Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels.C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation.Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology. |
| first_indexed | 2024-03-07T06:28:11Z |
| format | Journal article |
| id | oxford-uuid:f50c38e4-c1ae-4492-b86e-b0a5e9357a12 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:28:11Z |
| publishDate | 2015 |
| publisher | Future Medicine |
| record_format | dspace |
| spelling | oxford-uuid:f50c38e4-c1ae-4492-b86e-b0a5e9357a122022-03-27T12:24:21ZFunctionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f50c38e4-c1ae-4492-b86e-b0a5e9357a12EnglishSymplectic Elements at OxfordFuture Medicine2015Barkalina, NJones, CTownley, HCoward, KThis study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles.Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels.C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation.Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology. |
| spellingShingle | Barkalina, N Jones, C Townley, H Coward, K Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title | Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title_full | Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title_fullStr | Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title_full_unstemmed | Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title_short | Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. |
| title_sort | functionalization of mesoporous silica nanoparticles with a cell penetrating peptide to target mammalian sperm in vitro |
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