CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro.
An antibody reactive with CD38 revealed both phenotypic and functional heterogeneity amongst CD45RB(low) cells. Functional analysis of the CD38+ and CD38- fractions showed that the latter contained T cells which responded to recall antigens and produced high levels of cytokine in response to polyclo...
| Glavni autori: | , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
1998
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| _version_ | 1826305205031927808 |
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| author | Read, S Mauze, S Asseman, C Bean, A Coffman, R Powrie, F |
| author_facet | Read, S Mauze, S Asseman, C Bean, A Coffman, R Powrie, F |
| author_sort | Read, S |
| collection | OXFORD |
| description | An antibody reactive with CD38 revealed both phenotypic and functional heterogeneity amongst CD45RB(low) cells. Functional analysis of the CD38+ and CD38- fractions showed that the latter contained T cells which responded to recall antigens and produced high levels of cytokine in response to polyclonal stimulation. In contrast, the CD38+ population failed to proliferate or to produce detectable levels of cytokines. Despite appearing unresponsive, the CD38+ population significantly inhibited anti-CD3-induced proliferation and cytokine secretion by the reciprocal CD38- population. Immune suppression required stimulation through the TCR and was dependent on a physical interaction between regulatory and responding CD4+ populations. It did not involve killing of the responding T cells or secretion of IL-10 or TGF-beta. Despite some similarities there is no direct correlation between the in vitro suppression characteristic of the CD38+ CD45RB(low) subset and in vivo suppression which has been shown to be mediated by unseparated CD45RB(low) CD4+ T cells. However, these results demonstrate that two functionally distinct subsets of T cells reside within the antigen-exposed or CD45RB(low) CD4+ T cell population and are thus generated in vivo: (1) conventional memory T cells which proliferate and secrete cytokines in response to activation and (2) a population of regulatory T cells which inhibit T cell activation in vitro. Antibodies reactive with CD38 may provide a useful tool with which to study the role of these T cell subsets in the induction and regulation of the immune response. |
| first_indexed | 2024-03-07T06:29:21Z |
| format | Journal article |
| id | oxford-uuid:f5703b0d-6640-48ae-a58a-cadb143f5752 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:29:21Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:f5703b0d-6640-48ae-a58a-cadb143f57522022-03-27T12:27:18ZCD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f5703b0d-6640-48ae-a58a-cadb143f5752EnglishSymplectic Elements at Oxford1998Read, SMauze, SAsseman, CBean, ACoffman, RPowrie, FAn antibody reactive with CD38 revealed both phenotypic and functional heterogeneity amongst CD45RB(low) cells. Functional analysis of the CD38+ and CD38- fractions showed that the latter contained T cells which responded to recall antigens and produced high levels of cytokine in response to polyclonal stimulation. In contrast, the CD38+ population failed to proliferate or to produce detectable levels of cytokines. Despite appearing unresponsive, the CD38+ population significantly inhibited anti-CD3-induced proliferation and cytokine secretion by the reciprocal CD38- population. Immune suppression required stimulation through the TCR and was dependent on a physical interaction between regulatory and responding CD4+ populations. It did not involve killing of the responding T cells or secretion of IL-10 or TGF-beta. Despite some similarities there is no direct correlation between the in vitro suppression characteristic of the CD38+ CD45RB(low) subset and in vivo suppression which has been shown to be mediated by unseparated CD45RB(low) CD4+ T cells. However, these results demonstrate that two functionally distinct subsets of T cells reside within the antigen-exposed or CD45RB(low) CD4+ T cell population and are thus generated in vivo: (1) conventional memory T cells which proliferate and secrete cytokines in response to activation and (2) a population of regulatory T cells which inhibit T cell activation in vitro. Antibodies reactive with CD38 may provide a useful tool with which to study the role of these T cell subsets in the induction and regulation of the immune response. |
| spellingShingle | Read, S Mauze, S Asseman, C Bean, A Coffman, R Powrie, F CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title | CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title_full | CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title_fullStr | CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title_full_unstemmed | CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title_short | CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro. |
| title_sort | cd38 cd45rb low cd4 t cells a population of t cells with immune regulatory activities in vitro |
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