Expression of lectin-like transcript-1 in human tissues
<strong>Background:</strong> Receptor-ligand pairs of C-type lectin-like proteins have been shown to play an important role in cross talk between lymphocytes, as well as in immune responses within concrete tissues and structures, such as the skin or the germinal centres. The CD161-Lectin...
| Hlavní autoři: | , , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
F1000Research
2016
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| _version_ | 1826305247656542208 |
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| author | Llibre, A Garner, L Partridge, A Freeman, GJ Klenerman, P Willberg, CB |
| author_facet | Llibre, A Garner, L Partridge, A Freeman, GJ Klenerman, P Willberg, CB |
| author_sort | Llibre, A |
| collection | OXFORD |
| description | <strong>Background:</strong> Receptor-ligand pairs of C-type lectin-like proteins have been shown to play an important role in cross talk between lymphocytes, as well as in immune responses within concrete tissues and structures, such as the skin or the germinal centres. The CD161-Lectin-like Transcript 1 (LLT1) pair has gained particular attention in recent years, yet a detailed analysis of LLT1 distribution in human tissue is lacking. One reason for this is the limited availability and poor characterisation of anti-LLT1 antibodies. <strong>Methods:</strong> We assessed the staining capabilities of a novel anti-LLT1 antibody clone (2H7), both by immunohistochemistry and flow cytometry, showing its efficiency at LLT1 recognition in both settings. We then analysed LLT1 expression in a wide variety of human tissues. <strong>Results:</strong> We found LLT1 expression in circulating B cells and monocytes, but not in lung and liver-resident macrophages. We found strikingly high LLT1 expression in immune-privileged sites, such as the brain, placenta and testes, and confirmed the ability of LLT1 to inhibit NK cell function. <strong>Conclusions:</strong> Overall, this study contributes to the development of efficient tools for the study of LLT1. Moreover, its expression in different healthy human tissues and, particularly, in immune-privileged sites, establishes LLT1 as a good candidate as a regulator of immune responses. |
| first_indexed | 2024-03-07T06:30:01Z |
| format | Journal article |
| id | oxford-uuid:f5ac9d3c-3f17-49e6-bd33-4a42e41d2cdb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:30:01Z |
| publishDate | 2016 |
| publisher | F1000Research |
| record_format | dspace |
| spelling | oxford-uuid:f5ac9d3c-3f17-49e6-bd33-4a42e41d2cdb2022-03-27T12:28:59ZExpression of lectin-like transcript-1 in human tissuesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f5ac9d3c-3f17-49e6-bd33-4a42e41d2cdbEnglishSymplectic Elements at OxfordF1000Research2016Llibre, AGarner, LPartridge, AFreeman, GJKlenerman, PWillberg, CB<strong>Background:</strong> Receptor-ligand pairs of C-type lectin-like proteins have been shown to play an important role in cross talk between lymphocytes, as well as in immune responses within concrete tissues and structures, such as the skin or the germinal centres. The CD161-Lectin-like Transcript 1 (LLT1) pair has gained particular attention in recent years, yet a detailed analysis of LLT1 distribution in human tissue is lacking. One reason for this is the limited availability and poor characterisation of anti-LLT1 antibodies. <strong>Methods:</strong> We assessed the staining capabilities of a novel anti-LLT1 antibody clone (2H7), both by immunohistochemistry and flow cytometry, showing its efficiency at LLT1 recognition in both settings. We then analysed LLT1 expression in a wide variety of human tissues. <strong>Results:</strong> We found LLT1 expression in circulating B cells and monocytes, but not in lung and liver-resident macrophages. We found strikingly high LLT1 expression in immune-privileged sites, such as the brain, placenta and testes, and confirmed the ability of LLT1 to inhibit NK cell function. <strong>Conclusions:</strong> Overall, this study contributes to the development of efficient tools for the study of LLT1. Moreover, its expression in different healthy human tissues and, particularly, in immune-privileged sites, establishes LLT1 as a good candidate as a regulator of immune responses. |
| spellingShingle | Llibre, A Garner, L Partridge, A Freeman, GJ Klenerman, P Willberg, CB Expression of lectin-like transcript-1 in human tissues |
| title | Expression of lectin-like transcript-1 in human tissues |
| title_full | Expression of lectin-like transcript-1 in human tissues |
| title_fullStr | Expression of lectin-like transcript-1 in human tissues |
| title_full_unstemmed | Expression of lectin-like transcript-1 in human tissues |
| title_short | Expression of lectin-like transcript-1 in human tissues |
| title_sort | expression of lectin like transcript 1 in human tissues |
| work_keys_str_mv | AT llibrea expressionoflectinliketranscript1inhumantissues AT garnerl expressionoflectinliketranscript1inhumantissues AT partridgea expressionoflectinliketranscript1inhumantissues AT freemangj expressionoflectinliketranscript1inhumantissues AT klenermanp expressionoflectinliketranscript1inhumantissues AT willbergcb expressionoflectinliketranscript1inhumantissues |