The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis.
The epidermis of Arabidopsis wild-type primary roots, in which some cells grow hairs and others remain hairless in a position-dependent manner, has become an established model system to study cell differentiation. Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1998
|
| _version_ | 1826305260395692032 |
|---|---|
| author | Schneider, K Mathur, J Boudonck, K Wells, B Dolan, L Roberts, K |
| author_facet | Schneider, K Mathur, J Boudonck, K Wells, B Dolan, L Roberts, K |
| author_sort | Schneider, K |
| collection | OXFORD |
| description | The epidermis of Arabidopsis wild-type primary roots, in which some cells grow hairs and others remain hairless in a position-dependent manner, has become an established model system to study cell differentiation. Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutated, prevents the formation of hairs on primary roots and causes a seedling lethal phenotype. We have cloned the RHL1 gene by use of a T-DNA-tagged mutant and found that it encodes a protein that appears to be plant specific. The predicted RHL1 gene product is a small hydrophilic protein (38.9 kD) containing putative nuclear localization signals and shows no significant homology to any known amino acid sequence. We demonstrate that a 78-amino-acid sequence at its amino terminus is capable of directing an RHL1-GFP fusion protein to the nucleus. The RHL1 transcript is present throughout the wild-type plant and in suspension culture cells, but in very low amounts, suggesting a regulatory function for the RHL1 protein. Structural evidence suggests a role for the RHL1 gene product in the nucleolus. We have examined the genetic relationship between RHL1 and GL2, an inhibitor of root hair initiation in non-hair cells. Our molecular and genetic data with double mutants, together with the expression analysis of a GL2 promoter-GUS reporter gene construct, indicate that the RHL1 gene acts independently of GL2. |
| first_indexed | 2024-03-07T06:30:13Z |
| format | Journal article |
| id | oxford-uuid:f5bd9270-b3a0-4ec5-a72f-4205c4cb2b98 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:30:13Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:f5bd9270-b3a0-4ec5-a72f-4205c4cb2b982022-03-27T12:29:31ZThe ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f5bd9270-b3a0-4ec5-a72f-4205c4cb2b98EnglishSymplectic Elements at Oxford1998Schneider, KMathur, JBoudonck, KWells, BDolan, LRoberts, KThe epidermis of Arabidopsis wild-type primary roots, in which some cells grow hairs and others remain hairless in a position-dependent manner, has become an established model system to study cell differentiation. Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutated, prevents the formation of hairs on primary roots and causes a seedling lethal phenotype. We have cloned the RHL1 gene by use of a T-DNA-tagged mutant and found that it encodes a protein that appears to be plant specific. The predicted RHL1 gene product is a small hydrophilic protein (38.9 kD) containing putative nuclear localization signals and shows no significant homology to any known amino acid sequence. We demonstrate that a 78-amino-acid sequence at its amino terminus is capable of directing an RHL1-GFP fusion protein to the nucleus. The RHL1 transcript is present throughout the wild-type plant and in suspension culture cells, but in very low amounts, suggesting a regulatory function for the RHL1 protein. Structural evidence suggests a role for the RHL1 gene product in the nucleolus. We have examined the genetic relationship between RHL1 and GL2, an inhibitor of root hair initiation in non-hair cells. Our molecular and genetic data with double mutants, together with the expression analysis of a GL2 promoter-GUS reporter gene construct, indicate that the RHL1 gene acts independently of GL2. |
| spellingShingle | Schneider, K Mathur, J Boudonck, K Wells, B Dolan, L Roberts, K The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title | The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title_full | The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title_fullStr | The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title_full_unstemmed | The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title_short | The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis. |
| title_sort | root hairless 1 gene encodes a nuclear protein required for root hair initiation in arabidopsis |
| work_keys_str_mv | AT schneiderk theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT mathurj theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT boudonckk theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT wellsb theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT dolanl theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT robertsk theroothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT schneiderk roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT mathurj roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT boudonckk roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT wellsb roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT dolanl roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis AT robertsk roothairless1geneencodesanuclearproteinrequiredforroothairinitiationinarabidopsis |