Differential production of inflammatory chemokines by murine dendritic cell subsets.
Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naïve T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4+) or CD4(-)CD...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1797104181904932864 |
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| author | Proietto, A O'Keeffe, M Gartlan, K Wright, MD Shortman, K Wu, L Lahoud, M |
| author_facet | Proietto, A O'Keeffe, M Gartlan, K Wright, MD Shortman, K Wu, L Lahoud, M |
| author_sort | Proietto, A |
| collection | OXFORD |
| description | Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naïve T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4+) or CD4(-)CD8(+) (CD8+) subsets and the plasmacytoid DC (pDC), which have different immune regulatory functions. In this study, we investigated the differential expression of genes encoding the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes, and the secretion of these chemokines, among splenic DC subsets. These chemokine genes were expressed at higher levels by the splenic CD4+ and DN cDC subsets compared with the CD8+ cDC, in both the resting and activated states in vivo. Both the pDC and cDC subsets displayed increases in chemokine secretion in response to a range of toll-like receptor (TLR) stimuli in vitro. Whilst the pDC were the highest producers of Mip-1alpha and Mip-1beta in response to some TLR stimuli, the DN and CD4+ cDC subsets were the superior producers of Rantes. Overall, of the cDC, the CD4+ cDC produced all chemokines most efficiently, both at a basal level, and in response to most TLR stimuli. Thus, we report a new functional difference between the murine splenic cDC subsets, with the CD4+ cDC demonstrating the most efficient production of the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes. |
| first_indexed | 2024-03-07T06:30:15Z |
| format | Journal article |
| id | oxford-uuid:f5c01b4b-d915-4b82-8407-b615b17b4d9e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:30:15Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:f5c01b4b-d915-4b82-8407-b615b17b4d9e2022-03-27T12:29:38ZDifferential production of inflammatory chemokines by murine dendritic cell subsets.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f5c01b4b-d915-4b82-8407-b615b17b4d9eEnglishSymplectic Elements at Oxford2004Proietto, AO'Keeffe, MGartlan, KWright, MDShortman, KWu, LLahoud, MDendritic cells (DC) are efficient antigen presenting cells with the ability to activate naïve T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4+) or CD4(-)CD8(+) (CD8+) subsets and the plasmacytoid DC (pDC), which have different immune regulatory functions. In this study, we investigated the differential expression of genes encoding the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes, and the secretion of these chemokines, among splenic DC subsets. These chemokine genes were expressed at higher levels by the splenic CD4+ and DN cDC subsets compared with the CD8+ cDC, in both the resting and activated states in vivo. Both the pDC and cDC subsets displayed increases in chemokine secretion in response to a range of toll-like receptor (TLR) stimuli in vitro. Whilst the pDC were the highest producers of Mip-1alpha and Mip-1beta in response to some TLR stimuli, the DN and CD4+ cDC subsets were the superior producers of Rantes. Overall, of the cDC, the CD4+ cDC produced all chemokines most efficiently, both at a basal level, and in response to most TLR stimuli. Thus, we report a new functional difference between the murine splenic cDC subsets, with the CD4+ cDC demonstrating the most efficient production of the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes. |
| spellingShingle | Proietto, A O'Keeffe, M Gartlan, K Wright, MD Shortman, K Wu, L Lahoud, M Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title | Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title_full | Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title_fullStr | Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title_full_unstemmed | Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title_short | Differential production of inflammatory chemokines by murine dendritic cell subsets. |
| title_sort | differential production of inflammatory chemokines by murine dendritic cell subsets |
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