Regulation of activation induced deaminase (AID) by estrogen
Regulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expre...
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| Format: | Book section |
| Language: | English |
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Humana Press
2016
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| _version_ | 1826305421592231936 |
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| author | Pauklin, S |
| author_facet | Pauklin, S |
| author_sort | Pauklin, S |
| collection | OXFORD |
| description | Regulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expression and activity induced by estrogen, focusing on the isolation and preparation of cells for hormone treatment and the subsequent analysis of AID responsiveness to estrogen at the RNA level and for determining the regulation of AID activity via estrogen by analyzing Ig switch circle transcripts and mutations in switch region loci. |
| first_indexed | 2024-03-07T06:32:37Z |
| format | Book section |
| id | oxford-uuid:f688aa6f-7096-4112-b65f-d800fadc88b6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:32:37Z |
| publishDate | 2016 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:f688aa6f-7096-4112-b65f-d800fadc88b62022-03-27T12:35:42ZRegulation of activation induced deaminase (AID) by estrogenBook sectionhttp://purl.org/coar/resource_type/c_3248uuid:f688aa6f-7096-4112-b65f-d800fadc88b6EnglishSymplectic Elements at OxfordHumana Press2016Pauklin, SRegulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expression and activity induced by estrogen, focusing on the isolation and preparation of cells for hormone treatment and the subsequent analysis of AID responsiveness to estrogen at the RNA level and for determining the regulation of AID activity via estrogen by analyzing Ig switch circle transcripts and mutations in switch region loci. |
| spellingShingle | Pauklin, S Regulation of activation induced deaminase (AID) by estrogen |
| title | Regulation of activation induced deaminase (AID) by estrogen |
| title_full | Regulation of activation induced deaminase (AID) by estrogen |
| title_fullStr | Regulation of activation induced deaminase (AID) by estrogen |
| title_full_unstemmed | Regulation of activation induced deaminase (AID) by estrogen |
| title_short | Regulation of activation induced deaminase (AID) by estrogen |
| title_sort | regulation of activation induced deaminase aid by estrogen |
| work_keys_str_mv | AT pauklins regulationofactivationinduceddeaminaseaidbyestrogen |