Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum.
About 96% of the amino acid sequence of an alpha-tubulin from the slime mould Physarum polycephalum has been determined. Of 430 sequenced amino acids, 30 differ from the deduced amino acid sequence of a recently published alpha-tubulin complementary DNA from the plasmodial form of P. polycephalum. T...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1986
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| _version_ | 1797104365545193472 |
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| author | Singhofer-Wowra, M Little, M Clayton, L Dawson, P Gull, K |
| author_facet | Singhofer-Wowra, M Little, M Clayton, L Dawson, P Gull, K |
| author_sort | Singhofer-Wowra, M |
| collection | OXFORD |
| description | About 96% of the amino acid sequence of an alpha-tubulin from the slime mould Physarum polycephalum has been determined. Of 430 sequenced amino acids, 30 differ from the deduced amino acid sequence of a recently published alpha-tubulin complementary DNA from the plasmodial form of P. polycephalum. The myxamoebal alpha-tubulin differs from all other known alpha-tubulins in one of the last three C-terminal amino acids that are Gly-Glu-Tyr instead of the usual Glu-Glu-Tyr. These last three amino acids are preceded by 11 residues that appear to be particularly susceptible to mutation. No heterogeneity was found whilst sequencing the myxamoebal alpha-tubulin, indicating that only one type of alpha-tubulin is present in myxamoebae. This alpha-tubulin appears to be less conserved than the previously described plasmodial alpha-tubulin, supporting the hypothesis that the structural constraints on tubulin in axonemes have a significant effect on its rate of mutation. |
| first_indexed | 2024-03-07T06:32:48Z |
| format | Journal article |
| id | oxford-uuid:f698997f-a017-4136-adf8-01a4b2c47255 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:32:48Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:f698997f-a017-4136-adf8-01a4b2c472552022-03-27T12:36:12ZAmino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f698997f-a017-4136-adf8-01a4b2c47255EnglishSymplectic Elements at Oxford1986Singhofer-Wowra, MLittle, MClayton, LDawson, PGull, KAbout 96% of the amino acid sequence of an alpha-tubulin from the slime mould Physarum polycephalum has been determined. Of 430 sequenced amino acids, 30 differ from the deduced amino acid sequence of a recently published alpha-tubulin complementary DNA from the plasmodial form of P. polycephalum. The myxamoebal alpha-tubulin differs from all other known alpha-tubulins in one of the last three C-terminal amino acids that are Gly-Glu-Tyr instead of the usual Glu-Glu-Tyr. These last three amino acids are preceded by 11 residues that appear to be particularly susceptible to mutation. No heterogeneity was found whilst sequencing the myxamoebal alpha-tubulin, indicating that only one type of alpha-tubulin is present in myxamoebae. This alpha-tubulin appears to be less conserved than the previously described plasmodial alpha-tubulin, supporting the hypothesis that the structural constraints on tubulin in axonemes have a significant effect on its rate of mutation. |
| spellingShingle | Singhofer-Wowra, M Little, M Clayton, L Dawson, P Gull, K Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title | Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title_full | Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title_fullStr | Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title_full_unstemmed | Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title_short | Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. |
| title_sort | amino acid sequence data of alpha tubulin from myxamoebae of physarum polycephalum |
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