Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics
Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosci...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
American Society of Tropical Medicine and Hygiene
2020
|
| _version_ | 1797104479953223680 |
|---|---|
| author | Jang, IK Tyler, A Lyman, C Rek, JC Arinaitwe, E Adrama, H Murphy, M Imwong, M Proux, S Haohankhunnatham, W Barney, R Rashid, A Kalnoky, M Kahn, M Golden, A Nosten, F Greenhouse, B Gamboa, D Domingo, GJ |
| author_facet | Jang, IK Tyler, A Lyman, C Rek, JC Arinaitwe, E Adrama, H Murphy, M Imwong, M Proux, S Haohankhunnatham, W Barney, R Rashid, A Kalnoky, M Kahn, M Golden, A Nosten, F Greenhouse, B Gamboa, D Domingo, GJ |
| author_sort | Jang, IK |
| collection | OXFORD |
| description | Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), Plasmodium vivax -specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum. |
| first_indexed | 2024-03-07T06:34:24Z |
| format | Journal article |
| id | oxford-uuid:f71b6c36-6f2d-40e1-8317-bd56a3ee321a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:34:24Z |
| publishDate | 2020 |
| publisher | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | oxford-uuid:f71b6c36-6f2d-40e1-8317-bd56a3ee321a2022-03-27T12:40:22ZMultiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid DiagnosticsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f71b6c36-6f2d-40e1-8317-bd56a3ee321aEnglishSymplectic ElementsAmerican Society of Tropical Medicine and Hygiene2020Jang, IKTyler, ALyman, CRek, JCArinaitwe, EAdrama, HMurphy, MImwong, MProux, SHaohankhunnatham, WBarney, RRashid, AKalnoky, MKahn, MGolden, ANosten, FGreenhouse, BGamboa, DDomingo, GJMalaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), Plasmodium vivax -specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum. |
| spellingShingle | Jang, IK Tyler, A Lyman, C Rek, JC Arinaitwe, E Adrama, H Murphy, M Imwong, M Proux, S Haohankhunnatham, W Barney, R Rashid, A Kalnoky, M Kahn, M Golden, A Nosten, F Greenhouse, B Gamboa, D Domingo, GJ Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title | Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title_full | Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title_fullStr | Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title_full_unstemmed | Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title_short | Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics |
| title_sort | multiplex human malaria array quantifying antigens for malaria rapid diagnostics |
| work_keys_str_mv | AT jangik multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT tylera multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT lymanc multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT rekjc multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT arinaitwee multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT adramah multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT murphym multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT imwongm multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT prouxs multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT haohankhunnathamw multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT barneyr multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT rashida multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT kalnokym multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT kahnm multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT goldena multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT nostenf multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT greenhouseb multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT gamboad multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics AT domingogj multiplexhumanmalariaarrayquantifyingantigensformalariarapiddiagnostics |