Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS
<jats:p>Pyomyositis is a severe bacterial infection of skeletal muscle, commonly affecting children in tropical regions, predominantly caused by Staphylococcus aureus. To understand the contribution of bacterial genomic factors to pyomyositis, we conducted a genome-wide association study of S....
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences
2019
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| _version_ | 1826305564113633280 |
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| author | Young, BC Earle, SG Soeng, S Sar, P Kumar, V Hor, S Sar, V Bousfield, R Sanderson, ND Barker, L Stoesser, N Emary, KRW Parry, CM Nickerson, EK Turner, P Bowden, R Crook, DW Wyllie, DJ Day, NPJ Wilson, DJ Moore, CE |
| author_facet | Young, BC Earle, SG Soeng, S Sar, P Kumar, V Hor, S Sar, V Bousfield, R Sanderson, ND Barker, L Stoesser, N Emary, KRW Parry, CM Nickerson, EK Turner, P Bowden, R Crook, DW Wyllie, DJ Day, NPJ Wilson, DJ Moore, CE |
| author_sort | Young, BC |
| collection | OXFORD |
| description | <jats:p>Pyomyositis is a severe bacterial infection of skeletal muscle, commonly affecting children in tropical regions, predominantly caused by Staphylococcus aureus. To understand the contribution of bacterial genomic factors to pyomyositis, we conducted a genome-wide association study of S. aureus cultured from 101 children with pyomyositis and 417 children with asymptomatic nasal carriage attending the Angkor Hospital for Children, Cambodia. We found a strong relationship between bacterial genetic variation and pyomyositis, with estimated heritability 63.8% (95% CI 49.2-78.4%). The presence of the Panton-Valentine leucocidin (PVL) locus increased the odds of pyomyositis 130-fold (p=10-17.9). The signal of association mapped both to the PVL-coding sequence and the sequence immediately upstream. Together these regions explained over 99.9% of heritability (95% CI 93.5-100%). Our results establish staphylococcal pyomyositis, like tetanus and diphtheria, as critically dependent on a single toxin and demonstrate the potential for association studies to identify specific bacterial genes promoting severe human disease.</jats:p> |
| first_indexed | 2024-03-07T06:34:45Z |
| format | Journal article |
| id | oxford-uuid:f733ecf5-f65f-4efc-99e0-28800c98f867 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:34:45Z |
| publishDate | 2019 |
| publisher | eLife Sciences |
| record_format | dspace |
| spelling | oxford-uuid:f733ecf5-f65f-4efc-99e0-28800c98f8672022-03-27T12:41:17ZPanton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWASJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f733ecf5-f65f-4efc-99e0-28800c98f867EnglishSymplectic Elements at OxfordeLife Sciences2019Young, BCEarle, SGSoeng, SSar, PKumar, VHor, SSar, VBousfield, RSanderson, NDBarker, LStoesser, NEmary, KRWParry, CMNickerson, EKTurner, PBowden, RCrook, DWWyllie, DJDay, NPJWilson, DJMoore, CE<jats:p>Pyomyositis is a severe bacterial infection of skeletal muscle, commonly affecting children in tropical regions, predominantly caused by Staphylococcus aureus. To understand the contribution of bacterial genomic factors to pyomyositis, we conducted a genome-wide association study of S. aureus cultured from 101 children with pyomyositis and 417 children with asymptomatic nasal carriage attending the Angkor Hospital for Children, Cambodia. We found a strong relationship between bacterial genetic variation and pyomyositis, with estimated heritability 63.8% (95% CI 49.2-78.4%). The presence of the Panton-Valentine leucocidin (PVL) locus increased the odds of pyomyositis 130-fold (p=10-17.9). The signal of association mapped both to the PVL-coding sequence and the sequence immediately upstream. Together these regions explained over 99.9% of heritability (95% CI 93.5-100%). Our results establish staphylococcal pyomyositis, like tetanus and diphtheria, as critically dependent on a single toxin and demonstrate the potential for association studies to identify specific bacterial genes promoting severe human disease.</jats:p> |
| spellingShingle | Young, BC Earle, SG Soeng, S Sar, P Kumar, V Hor, S Sar, V Bousfield, R Sanderson, ND Barker, L Stoesser, N Emary, KRW Parry, CM Nickerson, EK Turner, P Bowden, R Crook, DW Wyllie, DJ Day, NPJ Wilson, DJ Moore, CE Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title | Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title_full | Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title_fullStr | Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title_full_unstemmed | Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title_short | Panton-Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS |
| title_sort | panton valentine leucocidin is the key determinant of staphylococcus aureus pyomyositis in a bacterial gwas |
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