Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs.
The second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) releases Ca(2+) from the acidic Ca(2+) stores of many organisms, including those of the sea urchin egg. We investigated whether the pH within the lumen of these acidic organelles changes in response to stimuli. Fertilization...
| Main Authors: | , |
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| Format: | Journal article |
| Jezik: | English |
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2007
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| _version_ | 1826305701566218240 |
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| author | Morgan, A Galione, A |
| author_facet | Morgan, A Galione, A |
| author_sort | Morgan, A |
| collection | OXFORD |
| description | The second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) releases Ca(2+) from the acidic Ca(2+) stores of many organisms, including those of the sea urchin egg. We investigated whether the pH within the lumen of these acidic organelles changes in response to stimuli. Fertilization activates the egg by Ca(2+) release dependent upon NAADP, and accordingly, we report that fertilization also alters organellar pH in a spatio-temporally complex manner. Upon sperm fusion, vesicles deep in the egg center slowly acidify, whereas cortical vesicles undergo a rapid alkalinization. The cortical vesicle alkalinization is independent of exocytosis and cytosolic pH but coincides with the NAADP-dependent fertilization Ca(2+) wave. Microinjection of NAADP mimicked the fertilization cortical response, suggesting that it occurred within NAADP-sensitive acidic Ca(2+) stores. Our data show that NAADP and physiological stimuli alter the pH within intracellular organelles and suggest that NAADP signals through pH as well as Ca(2+). |
| first_indexed | 2024-03-07T06:36:50Z |
| format | Journal article |
| id | oxford-uuid:f7f144a0-8acf-4c36-887c-794b3f19004a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:36:50Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:f7f144a0-8acf-4c36-887c-794b3f19004a2022-03-27T12:46:30ZFertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f7f144a0-8acf-4c36-887c-794b3f19004aEnglishSymplectic Elements at Oxford2007Morgan, AGalione, AThe second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) releases Ca(2+) from the acidic Ca(2+) stores of many organisms, including those of the sea urchin egg. We investigated whether the pH within the lumen of these acidic organelles changes in response to stimuli. Fertilization activates the egg by Ca(2+) release dependent upon NAADP, and accordingly, we report that fertilization also alters organellar pH in a spatio-temporally complex manner. Upon sperm fusion, vesicles deep in the egg center slowly acidify, whereas cortical vesicles undergo a rapid alkalinization. The cortical vesicle alkalinization is independent of exocytosis and cytosolic pH but coincides with the NAADP-dependent fertilization Ca(2+) wave. Microinjection of NAADP mimicked the fertilization cortical response, suggesting that it occurred within NAADP-sensitive acidic Ca(2+) stores. Our data show that NAADP and physiological stimuli alter the pH within intracellular organelles and suggest that NAADP signals through pH as well as Ca(2+). |
| spellingShingle | Morgan, A Galione, A Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title | Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title_full | Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title_fullStr | Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title_full_unstemmed | Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title_short | Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs. |
| title_sort | fertilization and nicotinic acid adenine dinucleotide phosphate induce ph changes in acidic ca 2 stores in sea urchin eggs |
| work_keys_str_mv | AT morgana fertilizationandnicotinicacidadeninedinucleotidephosphateinducephchangesinacidicca2storesinseaurchineggs AT galionea fertilizationandnicotinicacidadeninedinucleotidephosphateinducephchangesinacidicca2storesinseaurchineggs |