Analysis of B-cell immune tolerance induction using transgenic mice.
Over the past 15 yr, the use of transgenic mice has led to significant advances in our understanding of immunological tolerance. In a normal repertoire the number of B cells with a single antigen receptor specificity is very small, making the study of their fate difficult. In contrast, animals that...
| Autors principals: | , |
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| Format: | Journal article |
| Idioma: | English |
| Publicat: |
2004
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| _version_ | 1826305705867476992 |
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| author | Ferry, H Cornall, R |
| author_facet | Ferry, H Cornall, R |
| author_sort | Ferry, H |
| collection | OXFORD |
| description | Over the past 15 yr, the use of transgenic mice has led to significant advances in our understanding of immunological tolerance. In a normal repertoire the number of B cells with a single antigen receptor specificity is very small, making the study of their fate difficult. In contrast, animals that carry transgenes encoding rearranged immunoglobulin genes generate large numbers of B cells that, by the process of allelic exclusion, have an identical specificity. Exploitation of this effect has enabled the mechanisms involved in B-cell tolerance to be explored in some detail. In this review we use the hen egg lysozyme (HEL) model system to illustrate the generation and preparation of a transgene. In our example, we describe the generation of mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice. |
| first_indexed | 2024-03-07T06:36:54Z |
| format | Journal article |
| id | oxford-uuid:f7f62a19-adae-488e-9f78-24c9ed2b38a6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:36:54Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:f7f62a19-adae-488e-9f78-24c9ed2b38a62022-03-27T12:46:43ZAnalysis of B-cell immune tolerance induction using transgenic mice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f7f62a19-adae-488e-9f78-24c9ed2b38a6EnglishSymplectic Elements at Oxford2004Ferry, HCornall, ROver the past 15 yr, the use of transgenic mice has led to significant advances in our understanding of immunological tolerance. In a normal repertoire the number of B cells with a single antigen receptor specificity is very small, making the study of their fate difficult. In contrast, animals that carry transgenes encoding rearranged immunoglobulin genes generate large numbers of B cells that, by the process of allelic exclusion, have an identical specificity. Exploitation of this effect has enabled the mechanisms involved in B-cell tolerance to be explored in some detail. In this review we use the hen egg lysozyme (HEL) model system to illustrate the generation and preparation of a transgene. In our example, we describe the generation of mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice. |
| spellingShingle | Ferry, H Cornall, R Analysis of B-cell immune tolerance induction using transgenic mice. |
| title | Analysis of B-cell immune tolerance induction using transgenic mice. |
| title_full | Analysis of B-cell immune tolerance induction using transgenic mice. |
| title_fullStr | Analysis of B-cell immune tolerance induction using transgenic mice. |
| title_full_unstemmed | Analysis of B-cell immune tolerance induction using transgenic mice. |
| title_short | Analysis of B-cell immune tolerance induction using transgenic mice. |
| title_sort | analysis of b cell immune tolerance induction using transgenic mice |
| work_keys_str_mv | AT ferryh analysisofbcellimmunetoleranceinductionusingtransgenicmice AT cornallr analysisofbcellimmunetoleranceinductionusingtransgenicmice |