Structural basis and specificity of human otubain 1-mediated deubiquitination.

OTUB (otubain) 1 is a human deubiquitinating enzyme that is implicated in mediating lymphocyte antigen responsiveness, but whose molecular function is generally not well defined. A structural analysis of OTUB1 shows differences in accessibility to the active site and in surface properties of the sub...

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Hlavní autoři: Edelmann, M, Iphöfer, A, Akutsu, M, Altun, M, di Gleria, K, Kramer, H, Fiebiger, E, Dhe-Paganon, S, Kessler, B
Médium: Journal article
Jazyk:English
Vydáno: 2009
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author Edelmann, M
Iphöfer, A
Akutsu, M
Altun, M
di Gleria, K
Kramer, H
Fiebiger, E
Dhe-Paganon, S
Kessler, B
author_facet Edelmann, M
Iphöfer, A
Akutsu, M
Altun, M
di Gleria, K
Kramer, H
Fiebiger, E
Dhe-Paganon, S
Kessler, B
author_sort Edelmann, M
collection OXFORD
description OTUB (otubain) 1 is a human deubiquitinating enzyme that is implicated in mediating lymphocyte antigen responsiveness, but whose molecular function is generally not well defined. A structural analysis of OTUB1 shows differences in accessibility to the active site and in surface properties of the substrate-binding regions when compared with its close homologue, OTUB2, suggesting variations in regulatory mechanisms and substrate specificity. Biochemical analysis reveals that OTUB1 has a preference for cleaving Lys(48)-linked polyubiquitin chains over Lys(63)-linked polyubiquitin chains, and it is capable of cleaving NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8), but not SUMO (small ubiquitin-related modifier) 1/2/3 and ISG15 (interferon-stimulated gene 15) conjugates. A functional comparison of OTUB1 and OTUB2 indicated a differential reactivity towards ubiquitin-based active-site probes carrying a vinyl methyl ester, a 2-chloroethyl or a 2-bromoethyl group at the C-terminus. Mutational analysis suggested that a narrow P1' site, as observed in OTUB1, correlates with its ability to preferentially cleave Lys(48)-linked ubiquitin chains. Analysis of cellular interaction partners of OTUB1 by co-immunoprecipitation and MS/MS (tandem mass spectrometry) experiments demonstrated that FUS [fusion involved in t(12;6) in malignant liposarcoma; also known as TLS (translocation in liposarcoma) or CHOP (CCAAT/enhancer-binding protein homologous protein)] and RACK1 [receptor for activated kinase 1; also known as GNB2L1 (guanine-nucleotide-binding protein beta polypeptide 2-like 1)] are part of OTUB1-containing complexes, pointing towards a molecular function of this deubiquitinating enzyme in RNA processing and cell adhesion/morphology.
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spelling oxford-uuid:f8a0961b-8876-450c-be0e-0fc8c20c337d2022-03-27T12:51:44ZStructural basis and specificity of human otubain 1-mediated deubiquitination.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f8a0961b-8876-450c-be0e-0fc8c20c337dEnglishSymplectic Elements at Oxford2009Edelmann, MIphöfer, AAkutsu, MAltun, Mdi Gleria, KKramer, HFiebiger, EDhe-Paganon, SKessler, BOTUB (otubain) 1 is a human deubiquitinating enzyme that is implicated in mediating lymphocyte antigen responsiveness, but whose molecular function is generally not well defined. A structural analysis of OTUB1 shows differences in accessibility to the active site and in surface properties of the substrate-binding regions when compared with its close homologue, OTUB2, suggesting variations in regulatory mechanisms and substrate specificity. Biochemical analysis reveals that OTUB1 has a preference for cleaving Lys(48)-linked polyubiquitin chains over Lys(63)-linked polyubiquitin chains, and it is capable of cleaving NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8), but not SUMO (small ubiquitin-related modifier) 1/2/3 and ISG15 (interferon-stimulated gene 15) conjugates. A functional comparison of OTUB1 and OTUB2 indicated a differential reactivity towards ubiquitin-based active-site probes carrying a vinyl methyl ester, a 2-chloroethyl or a 2-bromoethyl group at the C-terminus. Mutational analysis suggested that a narrow P1' site, as observed in OTUB1, correlates with its ability to preferentially cleave Lys(48)-linked ubiquitin chains. Analysis of cellular interaction partners of OTUB1 by co-immunoprecipitation and MS/MS (tandem mass spectrometry) experiments demonstrated that FUS [fusion involved in t(12;6) in malignant liposarcoma; also known as TLS (translocation in liposarcoma) or CHOP (CCAAT/enhancer-binding protein homologous protein)] and RACK1 [receptor for activated kinase 1; also known as GNB2L1 (guanine-nucleotide-binding protein beta polypeptide 2-like 1)] are part of OTUB1-containing complexes, pointing towards a molecular function of this deubiquitinating enzyme in RNA processing and cell adhesion/morphology.
spellingShingle Edelmann, M
Iphöfer, A
Akutsu, M
Altun, M
di Gleria, K
Kramer, H
Fiebiger, E
Dhe-Paganon, S
Kessler, B
Structural basis and specificity of human otubain 1-mediated deubiquitination.
title Structural basis and specificity of human otubain 1-mediated deubiquitination.
title_full Structural basis and specificity of human otubain 1-mediated deubiquitination.
title_fullStr Structural basis and specificity of human otubain 1-mediated deubiquitination.
title_full_unstemmed Structural basis and specificity of human otubain 1-mediated deubiquitination.
title_short Structural basis and specificity of human otubain 1-mediated deubiquitination.
title_sort structural basis and specificity of human otubain 1 mediated deubiquitination
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