Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules.
The chemical labeling of biomolecules continues to be an important tool for the study of their function and cellular fate. Attention is increasingly focused on labeling of biomolecules in living cells, since cell lysis introduces many artefacts. In addition, with the advances in biocompatible synthe...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2005
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| _version_ | 1826305854381490176 |
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| author | van Swieten, P Leeuwenburgh, M Kessler, B Overkleeft, H |
| author_facet | van Swieten, P Leeuwenburgh, M Kessler, B Overkleeft, H |
| author_sort | van Swieten, P |
| collection | OXFORD |
| description | The chemical labeling of biomolecules continues to be an important tool for the study of their function and cellular fate. Attention is increasingly focused on labeling of biomolecules in living cells, since cell lysis introduces many artefacts. In addition, with the advances in biocompatible synthetic organic chemistry, a whole new field of opportunity has opened up, affording high diversity in the nature of the label as well as a choice of ligation reactions. In recent years, several different two-step labeling strategies have emerged. These rely on the introduction of a bioorthogonal attachment site into a biomolecule, then ligation of a reporter molecule to this site using bioorthogonal organic chemistry. This Perspective focuses on these techniques, their implications and future directions. |
| first_indexed | 2024-03-07T06:39:10Z |
| format | Journal article |
| id | oxford-uuid:f8b003fc-2dcb-4f2d-a507-9db440e6f2e5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:39:10Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:f8b003fc-2dcb-4f2d-a507-9db440e6f2e52022-03-27T12:52:10ZBioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f8b003fc-2dcb-4f2d-a507-9db440e6f2e5EnglishSymplectic Elements at Oxford2005van Swieten, PLeeuwenburgh, MKessler, BOverkleeft, HThe chemical labeling of biomolecules continues to be an important tool for the study of their function and cellular fate. Attention is increasingly focused on labeling of biomolecules in living cells, since cell lysis introduces many artefacts. In addition, with the advances in biocompatible synthetic organic chemistry, a whole new field of opportunity has opened up, affording high diversity in the nature of the label as well as a choice of ligation reactions. In recent years, several different two-step labeling strategies have emerged. These rely on the introduction of a bioorthogonal attachment site into a biomolecule, then ligation of a reporter molecule to this site using bioorthogonal organic chemistry. This Perspective focuses on these techniques, their implications and future directions. |
| spellingShingle | van Swieten, P Leeuwenburgh, M Kessler, B Overkleeft, H Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title | Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title_full | Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title_fullStr | Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title_full_unstemmed | Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title_short | Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules. |
| title_sort | bioorthogonal organic chemistry in living cells novel strategies for labeling biomolecules |
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