Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding.
The unique DNA-binding properties of distinct NF-κB dimers influence the selective regulation of NF-κB target genes. To more thoroughly investigate these dimer-specific differences, we combined protein-binding microarrays and surface plasmon resonance to evaluate DNA sites recognized by eight differ...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797104862297587712 |
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| author | Siggers, T Chang, AB Teixeira, A Wong, D Williams, K Ahmed, B Ragoussis, J Udalova, I Smale, S Bulyk, M |
| author_facet | Siggers, T Chang, AB Teixeira, A Wong, D Williams, K Ahmed, B Ragoussis, J Udalova, I Smale, S Bulyk, M |
| author_sort | Siggers, T |
| collection | OXFORD |
| description | The unique DNA-binding properties of distinct NF-κB dimers influence the selective regulation of NF-κB target genes. To more thoroughly investigate these dimer-specific differences, we combined protein-binding microarrays and surface plasmon resonance to evaluate DNA sites recognized by eight different NF-κB dimers. We observed three distinct binding-specificity classes and clarified mechanisms by which dimers might regulate distinct sets of genes. We identified many new nontraditional NF-κB binding site (κB site) sequences and highlight the plasticity of NF-κB dimers in recognizing κB sites with a single consensus half-site. This study provides a database that can be used in efforts to identify NF-κB target sites and uncover gene regulatory circuitry. |
| first_indexed | 2024-03-07T06:39:31Z |
| format | Journal article |
| id | oxford-uuid:f8c87290-ae27-4dda-909f-0ccaaeca6a9a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:39:31Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:f8c87290-ae27-4dda-909f-0ccaaeca6a9a2022-03-27T12:53:06ZPrinciples of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f8c87290-ae27-4dda-909f-0ccaaeca6a9aEnglishSymplectic Elements at Oxford2012Siggers, TChang, ABTeixeira, AWong, DWilliams, KAhmed, BRagoussis, JUdalova, ISmale, SBulyk, MThe unique DNA-binding properties of distinct NF-κB dimers influence the selective regulation of NF-κB target genes. To more thoroughly investigate these dimer-specific differences, we combined protein-binding microarrays and surface plasmon resonance to evaluate DNA sites recognized by eight different NF-κB dimers. We observed three distinct binding-specificity classes and clarified mechanisms by which dimers might regulate distinct sets of genes. We identified many new nontraditional NF-κB binding site (κB site) sequences and highlight the plasticity of NF-κB dimers in recognizing κB sites with a single consensus half-site. This study provides a database that can be used in efforts to identify NF-κB target sites and uncover gene regulatory circuitry. |
| spellingShingle | Siggers, T Chang, AB Teixeira, A Wong, D Williams, K Ahmed, B Ragoussis, J Udalova, I Smale, S Bulyk, M Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title | Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title_full | Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title_fullStr | Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title_full_unstemmed | Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title_short | Principles of dimer-specific gene regulation revealed by a comprehensive characterization of NF-κB family DNA binding. |
| title_sort | principles of dimer specific gene regulation revealed by a comprehensive characterization of nf κb family dna binding |
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