Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway
The oxygen sensitive prolyl-hydroxylase domain enzymes (PHDs) and the asparagines hydroxylase (FIH, factor inhibiting HIF) regulate the transcriptional activity of HIFs. We assessed the expression of these enzymes in a series of 73 non-small cell lung carcinomas (NSCLC). A direct association of PHDs...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2008
|
| _version_ | 1797104863119671296 |
|---|---|
| author | Giatromanolaki, A Koukourakis, M Pezzella, F Turley, H Sivridis, E Bouros, D Bougioukas, G Harris, A Gatter, K |
| author_facet | Giatromanolaki, A Koukourakis, M Pezzella, F Turley, H Sivridis, E Bouros, D Bougioukas, G Harris, A Gatter, K |
| author_sort | Giatromanolaki, A |
| collection | OXFORD |
| description | The oxygen sensitive prolyl-hydroxylase domain enzymes (PHDs) and the asparagines hydroxylase (FIH, factor inhibiting HIF) regulate the transcriptional activity of HIFs. We assessed the expression of these enzymes in a series of 73 non-small cell lung carcinomas (NSCLC). A direct association of PHDs with FIH and of the PHDs/FIH with HIFs expression was noted. Thirty three of 73 cases had high HIF/PHD expression, predicting intense HIF activity; 19/73 cases had low HIF and high PHD expression mimicking the normal bronchial pattern; and 18/73 cases had low HIF/PHD (inactive HIF pathway). High lactate dehydrogenase LDH5 expression was noted in cases with high HIF/PHD phenotype. The value of such a classification in defining different metabolic phenotypes of NSCLC deserves further evaluation. © 2007 Elsevier Ireland Ltd. |
| first_indexed | 2024-03-07T06:39:32Z |
| format | Journal article |
| id | oxford-uuid:f8c8bc3a-363b-495b-87fe-b4742e4535b3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:39:32Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:f8c8bc3a-363b-495b-87fe-b4742e4535b32022-03-27T12:53:08ZExpression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathwayJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f8c8bc3a-363b-495b-87fe-b4742e4535b3EnglishSymplectic Elements at Oxford2008Giatromanolaki, AKoukourakis, MPezzella, FTurley, HSivridis, EBouros, DBougioukas, GHarris, AGatter, KThe oxygen sensitive prolyl-hydroxylase domain enzymes (PHDs) and the asparagines hydroxylase (FIH, factor inhibiting HIF) regulate the transcriptional activity of HIFs. We assessed the expression of these enzymes in a series of 73 non-small cell lung carcinomas (NSCLC). A direct association of PHDs with FIH and of the PHDs/FIH with HIFs expression was noted. Thirty three of 73 cases had high HIF/PHD expression, predicting intense HIF activity; 19/73 cases had low HIF and high PHD expression mimicking the normal bronchial pattern; and 18/73 cases had low HIF/PHD (inactive HIF pathway). High lactate dehydrogenase LDH5 expression was noted in cases with high HIF/PHD phenotype. The value of such a classification in defining different metabolic phenotypes of NSCLC deserves further evaluation. © 2007 Elsevier Ireland Ltd. |
| spellingShingle | Giatromanolaki, A Koukourakis, M Pezzella, F Turley, H Sivridis, E Bouros, D Bougioukas, G Harris, A Gatter, K Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title | Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title_full | Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title_fullStr | Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title_full_unstemmed | Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title_short | Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway |
| title_sort | expression of prolyl hydroxylases phd 1 2 and 3 and of the asparagine hydroxylase fih in non small cell lung cancer relates to an activated hif pathway |
| work_keys_str_mv | AT giatromanolakia expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT koukourakism expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT pezzellaf expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT turleyh expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT sivridise expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT bourosd expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT bougioukasg expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT harrisa expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway AT gatterk expressionofprolylhydroxylasesphd12and3andoftheasparaginehydroxylasefihinnonsmallcelllungcancerrelatestoanactivatedhifpathway |