Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Royal Society of Chemistry
2022
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| _version_ | 1826309944640536576 |
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| author | Trumpp, M Oliveras, A Gonschior, H Ast, J Hodson, DJ Knaus, P Lehmann, M Birol, M Broichhagen, J |
| author_facet | Trumpp, M Oliveras, A Gonschior, H Ast, J Hodson, DJ Knaus, P Lehmann, M Birol, M Broichhagen, J |
| author_sort | Trumpp, M |
| collection | OXFORD |
| description | Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy. |
| first_indexed | 2024-03-07T07:43:15Z |
| format | Journal article |
| id | oxford-uuid:f95380bd-1745-40e2-8351-3727fe65bda5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:43:15Z |
| publishDate | 2022 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:f95380bd-1745-40e2-8351-3727fe65bda52023-05-11T15:16:51ZEnzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f95380bd-1745-40e2-8351-3727fe65bda5EnglishSymplectic ElementsRoyal Society of Chemistry2022Trumpp, MOliveras, AGonschior, HAst, JHodson, DJKnaus, PLehmann, MBirol, MBroichhagen, JHerein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy. |
| spellingShingle | Trumpp, M Oliveras, A Gonschior, H Ast, J Hodson, DJ Knaus, P Lehmann, M Birol, M Broichhagen, J Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title_full | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title_fullStr | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title_full_unstemmed | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title_short | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| title_sort | enzyme self label bound atto700 in single molecule and super resolution microscopy |
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