Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry.
Wavefront aberrations caused by the refractive index structure of the specimen are known to compromise signal intensity and three-dimensional resolution in confocal and multiphoton microscopy. However, adaptive optics can measure and correct specimen-induced aberrations. For the design of an adaptiv...
| Päätekijät: | , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
2004
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| _version_ | 1826306175325437952 |
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| author | Schwertner, M Booth, M Wilson, T |
| author_facet | Schwertner, M Booth, M Wilson, T |
| author_sort | Schwertner, M |
| collection | OXFORD |
| description | Wavefront aberrations caused by the refractive index structure of the specimen are known to compromise signal intensity and three-dimensional resolution in confocal and multiphoton microscopy. However, adaptive optics can measure and correct specimen-induced aberrations. For the design of an adaptive optics system, information on the type and amount of the aberration is required. We have previously described an interferometric set-up capable of measuring specimen-induced aberrations and a method for the extraction of the Zernike mode content. In this paper we have modelled specimen-induced aberrations caused by spherical and cylindrical objects using a ray tracing method. The Zernike mode content of the wavefronts was then extracted from the simulated wavefronts and compared with experimental results. Aberrations for a simple model of an oocyte cell consisting of two spherical regions and for a model of a well-characterized optical fibre are calculated. This simple model gave Zernike mode data that are in good agreement with experimental results. |
| first_indexed | 2024-03-07T06:43:57Z |
| format | Journal article |
| id | oxford-uuid:fa3e2950-c373-45c8-a177-91e5a65b0d4d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:43:57Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:fa3e2950-c373-45c8-a177-91e5a65b0d4d2022-03-27T13:04:12ZSimulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fa3e2950-c373-45c8-a177-91e5a65b0d4dEnglishSymplectic Elements at Oxford2004Schwertner, MBooth, MWilson, TWavefront aberrations caused by the refractive index structure of the specimen are known to compromise signal intensity and three-dimensional resolution in confocal and multiphoton microscopy. However, adaptive optics can measure and correct specimen-induced aberrations. For the design of an adaptive optics system, information on the type and amount of the aberration is required. We have previously described an interferometric set-up capable of measuring specimen-induced aberrations and a method for the extraction of the Zernike mode content. In this paper we have modelled specimen-induced aberrations caused by spherical and cylindrical objects using a ray tracing method. The Zernike mode content of the wavefronts was then extracted from the simulated wavefronts and compared with experimental results. Aberrations for a simple model of an oocyte cell consisting of two spherical regions and for a model of a well-characterized optical fibre are calculated. This simple model gave Zernike mode data that are in good agreement with experimental results. |
| spellingShingle | Schwertner, M Booth, M Wilson, T Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title | Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title_full | Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title_fullStr | Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title_full_unstemmed | Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title_short | Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. |
| title_sort | simulation of specimen induced aberrations for objects with spherical and cylindrical symmetry |
| work_keys_str_mv | AT schwertnerm simulationofspecimeninducedaberrationsforobjectswithsphericalandcylindricalsymmetry AT boothm simulationofspecimeninducedaberrationsforobjectswithsphericalandcylindricalsymmetry AT wilsont simulationofspecimeninducedaberrationsforobjectswithsphericalandcylindricalsymmetry |