Zooming-in on the proteome: very narrow-range immobilised pH gradients reveal more protein species and isoforms.
Two-dimensional gel electrophoresis (2-DE) enables separation of complex mixtures of proteins on a single polyacrylamide gel according to isoelectric point, molecular weight, solubility, and relative abundance. For this reason, 2-DE together with mass spectrometry (MS) has become a key technology in...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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