A compact light-sheet microscope for the study of the mammalian central nervous system
Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inv...
| Main Authors: | , , , , |
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| Format: | Journal article |
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Nature Publishing Group
2016
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| _version_ | 1826306344696676352 |
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| author | Yang, Z Haslehurst, P Scott, S Emptage, N Dholakia, K |
| author_facet | Yang, Z Haslehurst, P Scott, S Emptage, N Dholakia, K |
| author_sort | Yang, Z |
| collection | OXFORD |
| description | Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community. |
| first_indexed | 2024-03-07T06:46:32Z |
| format | Journal article |
| id | oxford-uuid:fb0936cf-566b-4d86-9c06-7794b3d0aaec |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:46:32Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:fb0936cf-566b-4d86-9c06-7794b3d0aaec2022-03-27T13:10:50ZA compact light-sheet microscope for the study of the mammalian central nervous systemJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fb0936cf-566b-4d86-9c06-7794b3d0aaecSymplectic Elements at OxfordNature Publishing Group2016Yang, ZHaslehurst, PScott, SEmptage, NDholakia, KInvestigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community. |
| spellingShingle | Yang, Z Haslehurst, P Scott, S Emptage, N Dholakia, K A compact light-sheet microscope for the study of the mammalian central nervous system |
| title | A compact light-sheet microscope for the study of the mammalian central nervous system |
| title_full | A compact light-sheet microscope for the study of the mammalian central nervous system |
| title_fullStr | A compact light-sheet microscope for the study of the mammalian central nervous system |
| title_full_unstemmed | A compact light-sheet microscope for the study of the mammalian central nervous system |
| title_short | A compact light-sheet microscope for the study of the mammalian central nervous system |
| title_sort | compact light sheet microscope for the study of the mammalian central nervous system |
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