Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes
Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are rev...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Shared Science Publishers
2019
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| _version_ | 1826306514733760512 |
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| author | Klein, HL Ang, KKH Arkin, MR Beckwitt, EC Chang, Y-H Fan, J Kwon, Y Morten, MJ Mukherjee, S Pambos, OJ Sayyed, H Thrall, ES Vieira-Da-Rocha, JP Wang, Q Wang, S Yeh, H-Y Biteen, JS Chi, P Heyer, W-D Kapanidis, AN Loparo, JJ Strick, TR Sung, P Van Houten, B Niu, H Rothenberg, E |
| author_facet | Klein, HL Ang, KKH Arkin, MR Beckwitt, EC Chang, Y-H Fan, J Kwon, Y Morten, MJ Mukherjee, S Pambos, OJ Sayyed, H Thrall, ES Vieira-Da-Rocha, JP Wang, Q Wang, S Yeh, H-Y Biteen, JS Chi, P Heyer, W-D Kapanidis, AN Loparo, JJ Strick, TR Sung, P Van Houten, B Niu, H Rothenberg, E |
| author_sort | Klein, HL |
| collection | OXFORD |
| description | Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. |
| first_indexed | 2024-03-07T06:49:06Z |
| format | Journal article |
| id | oxford-uuid:fbe5d1c7-ad0a-4d32-ba51-6edece8fdf97 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:49:06Z |
| publishDate | 2019 |
| publisher | Shared Science Publishers |
| record_format | dspace |
| spelling | oxford-uuid:fbe5d1c7-ad0a-4d32-ba51-6edece8fdf972022-03-27T13:17:04ZGuidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fbe5d1c7-ad0a-4d32-ba51-6edece8fdf97EnglishSymplectic Elements at OxfordShared Science Publishers2019Klein, HLAng, KKHArkin, MRBeckwitt, ECChang, Y-HFan, JKwon, YMorten, MJMukherjee, SPambos, OJSayyed, HThrall, ESVieira-Da-Rocha, JPWang, QWang, SYeh, H-YBiteen, JSChi, PHeyer, W-DKapanidis, ANLoparo, JJStrick, TRSung, PVan Houten, BNiu, HRothenberg, EGenomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. |
| spellingShingle | Klein, HL Ang, KKH Arkin, MR Beckwitt, EC Chang, Y-H Fan, J Kwon, Y Morten, MJ Mukherjee, S Pambos, OJ Sayyed, H Thrall, ES Vieira-Da-Rocha, JP Wang, Q Wang, S Yeh, H-Y Biteen, JS Chi, P Heyer, W-D Kapanidis, AN Loparo, JJ Strick, TR Sung, P Van Houten, B Niu, H Rothenberg, E Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title | Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title_full | Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title_fullStr | Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title_full_unstemmed | Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title_short | Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes |
| title_sort | guidelines for dna recombination and repair studies mechanistic assays of dna repair processes |
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