Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new S...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2006
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| _version_ | 1797105629384409088 |
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| author | Li, S Sandercock, A Conduit, P Robinson, C Williams, R Kilmartin, J |
| author_facet | Li, S Sandercock, A Conduit, P Robinson, C Williams, R Kilmartin, J |
| author_sort | Li, S |
| collection | OXFORD |
| description | Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly. |
| first_indexed | 2024-03-07T06:50:07Z |
| format | Journal article |
| id | oxford-uuid:fc4464f9-b6dc-4c8c-9579-a666f1d2a1b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:50:07Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:fc4464f9-b6dc-4c8c-9579-a666f1d2a1b42022-03-27T13:19:34ZStructural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fc4464f9-b6dc-4c8c-9579-a666f1d2a1b4EnglishSymplectic Elements at Oxford2006Li, SSandercock, AConduit, PRobinson, CWilliams, RKilmartin, JCentrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly. |
| spellingShingle | Li, S Sandercock, A Conduit, P Robinson, C Williams, R Kilmartin, J Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title_full | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title_fullStr | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title_full_unstemmed | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title_short | Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. |
| title_sort | structural role of sfi1p centrin filaments in budding yeast spindle pole body duplication |
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