Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts.
A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleoca...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2002
|
| _version_ | 1797105648990683136 |
|---|---|
| author | Sláviková, M Kocáková, P Slovák, M Vancová, I Hajnická, V Gasperík, J Fuchsberger, N Nuttall, P |
| author_facet | Sláviková, M Kocáková, P Slovák, M Vancová, I Hajnická, V Gasperík, J Fuchsberger, N Nuttall, P |
| author_sort | Sláviková, M |
| collection | OXFORD |
| description | A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear. |
| first_indexed | 2024-03-07T06:50:20Z |
| format | Journal article |
| id | oxford-uuid:fc594761-66af-4c3c-b8fc-3d038177f00b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:50:20Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:fc594761-66af-4c3c-b8fc-3d038177f00b2022-03-27T13:19:59ZVesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fc594761-66af-4c3c-b8fc-3d038177f00bEnglishSymplectic Elements at Oxford2002Sláviková, MKocáková, PSlovák, MVancová, IHajnická, VGasperík, JFuchsberger, NNuttall, PA salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear. |
| spellingShingle | Sláviková, M Kocáková, P Slovák, M Vancová, I Hajnická, V Gasperík, J Fuchsberger, N Nuttall, P Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title | Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title_full | Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title_fullStr | Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title_full_unstemmed | Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title_short | Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts. |
| title_sort | vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts |
| work_keys_str_mv | AT slavikovam vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT kocakovap vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT slovakm vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT vancovai vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT hajnickav vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT gasperikj vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT fuchsbergern vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts AT nuttallp vesicularstomatitisvirusnucleocapsidproteinproductionincellstreatedwithselectedfastproteinliquidchromatographyfractionsofticksalivaryglandextracts |