Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy.
The Krebs cycle plays a fundamental role in cardiac energy production and is often implicated in the energetic imbalance characteristic of heart disease. In this study, we measured Krebs cycle flux in real time in perfused rat hearts using hyperpolarized magnetic resonance spectroscopy (MRS). [2-(13...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| _version_ | 1826306600955019264 |
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| author | Schroeder, M Atherton, H Ball, DR Cole, M Heather, L Griffin, J Clarke, K Radda, G Tyler, D |
| author_facet | Schroeder, M Atherton, H Ball, DR Cole, M Heather, L Griffin, J Clarke, K Radda, G Tyler, D |
| author_sort | Schroeder, M |
| collection | OXFORD |
| description | The Krebs cycle plays a fundamental role in cardiac energy production and is often implicated in the energetic imbalance characteristic of heart disease. In this study, we measured Krebs cycle flux in real time in perfused rat hearts using hyperpolarized magnetic resonance spectroscopy (MRS). [2-(13)C]Pyruvate was hyperpolarized and infused into isolated perfused hearts in both healthy and postischemic metabolic states. We followed the enzymatic conversion of pyruvate to lactate, acetylcarnitine, citrate, and glutamate with 1 s temporal resolution. The appearance of (13)C-labeled glutamate was delayed compared with that of other metabolites, indicating that Krebs cycle flux can be measured directly. The production of (13)C-labeled citrate and glutamate was decreased postischemia, as opposed to lactate, which was significantly elevated. These results showed that the control and fluxes of the Krebs cycle in heart disease can be studied using hyperpolarized [2-(13)C]pyruvate. |
| first_indexed | 2024-03-07T06:50:25Z |
| format | Journal article |
| id | oxford-uuid:fc6098eb-94ac-4064-91df-fb86b75be282 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:50:25Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:fc6098eb-94ac-4064-91df-fb86b75be2822022-03-27T13:20:09ZReal-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fc6098eb-94ac-4064-91df-fb86b75be282EnglishSymplectic Elements at Oxford2009Schroeder, MAtherton, HBall, DRCole, MHeather, LGriffin, JClarke, KRadda, GTyler, DThe Krebs cycle plays a fundamental role in cardiac energy production and is often implicated in the energetic imbalance characteristic of heart disease. In this study, we measured Krebs cycle flux in real time in perfused rat hearts using hyperpolarized magnetic resonance spectroscopy (MRS). [2-(13)C]Pyruvate was hyperpolarized and infused into isolated perfused hearts in both healthy and postischemic metabolic states. We followed the enzymatic conversion of pyruvate to lactate, acetylcarnitine, citrate, and glutamate with 1 s temporal resolution. The appearance of (13)C-labeled glutamate was delayed compared with that of other metabolites, indicating that Krebs cycle flux can be measured directly. The production of (13)C-labeled citrate and glutamate was decreased postischemia, as opposed to lactate, which was significantly elevated. These results showed that the control and fluxes of the Krebs cycle in heart disease can be studied using hyperpolarized [2-(13)C]pyruvate. |
| spellingShingle | Schroeder, M Atherton, H Ball, DR Cole, M Heather, L Griffin, J Clarke, K Radda, G Tyler, D Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title | Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title_full | Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title_fullStr | Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title_full_unstemmed | Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title_short | Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy. |
| title_sort | real time assessment of krebs cycle metabolism using hyperpolarized 13c magnetic resonance spectroscopy |
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