Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction
Cyclization of short hairpin oligonucleotides (ODNs) containing unactivated alkynes and alkyl azides produces triazole-linked products slowly in the absence of Cu I catalysis. The factors affecting the rate of this cycloaddition reaction have been investigated on a variety of...
| Main Authors: | , |
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| פורמט: | Journal article |
| שפה: | English |
| יצא לאור: |
2010
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| _version_ | 1826306643670859776 |
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| author | El-Sagheer, A Brown, T |
| author_facet | El-Sagheer, A Brown, T |
| author_sort | El-Sagheer, A |
| collection | OXFORD |
| description | Cyclization of short hairpin oligonucleotides (ODNs) containing unactivated alkynes and alkyl azides produces triazole-linked products slowly in the absence of Cu I catalysis. The factors affecting the rate of this cycloaddition reaction have been investigated on a variety of hairpin sequences. The reaction proceeds best at intermediate concentrations of sodium chloride, but is inhibited by phosphate and Tris buffers. It is accelerated by increasing the temperature provided that Watson-Crick base pairing can occur. The rationale for these observations is discussed. © 2010 IUPAC. |
| first_indexed | 2024-03-07T06:51:05Z |
| format | Journal article |
| id | oxford-uuid:fc935599-a68b-49dc-8697-3bd4f0ddd8d3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:51:05Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:fc935599-a68b-49dc-8697-3bd4f0ddd8d32022-03-27T13:21:52ZFactors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reactionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fc935599-a68b-49dc-8697-3bd4f0ddd8d3EnglishSymplectic Elements at Oxford2010El-Sagheer, ABrown, TCyclization of short hairpin oligonucleotides (ODNs) containing unactivated alkynes and alkyl azides produces triazole-linked products slowly in the absence of Cu I catalysis. The factors affecting the rate of this cycloaddition reaction have been investigated on a variety of hairpin sequences. The reaction proceeds best at intermediate concentrations of sodium chloride, but is inhibited by phosphate and Tris buffers. It is accelerated by increasing the temperature provided that Watson-Crick base pairing can occur. The rationale for these observations is discussed. © 2010 IUPAC. |
| spellingShingle | El-Sagheer, A Brown, T Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title | Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title_full | Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title_fullStr | Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title_full_unstemmed | Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title_short | Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction |
| title_sort | factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne azide cycloaddition aac reaction |
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