Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment.
Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II correspon...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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| _version_ | 1797105765872304128 |
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| author | Wain, J Hoa, N Chinh, N Vinh, H Everett, M Diep, T Day, N Solomon, T White, N Piddock, L Parry, C |
| author_facet | Wain, J Hoa, N Chinh, N Vinh, H Everett, M Diep, T Day, N Solomon, T White, N Piddock, L Parry, C |
| author_sort | Wain, J |
| collection | OXFORD |
| description | Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P < .001). Six (33.3%) of 18 NARST infections required retreatment, whereas 1 (0.8%) of 132 infections due to susceptible strains required retreatment (relative risk = 44; 95% confidence interval = 5.6-345; P < .0001). We recommend that short courses of quinolones not be used in patients infected with NARST. |
| first_indexed | 2024-03-07T06:52:03Z |
| format | Journal article |
| id | oxford-uuid:fce08969-5054-448a-82e0-65630c3a8fa4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:52:03Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:fce08969-5054-448a-82e0-65630c3a8fa42022-03-27T13:24:29ZQuinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fce08969-5054-448a-82e0-65630c3a8fa4EnglishSymplectic Elements at Oxford1997Wain, JHoa, NChinh, NVinh, HEverett, MDiep, TDay, NSolomon, TWhite, NPiddock, LParry, CNalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P < .001). Six (33.3%) of 18 NARST infections required retreatment, whereas 1 (0.8%) of 132 infections due to susceptible strains required retreatment (relative risk = 44; 95% confidence interval = 5.6-345; P < .0001). We recommend that short courses of quinolones not be used in patients infected with NARST. |
| spellingShingle | Wain, J Hoa, N Chinh, N Vinh, H Everett, M Diep, T Day, N Solomon, T White, N Piddock, L Parry, C Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title | Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title_full | Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title_fullStr | Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title_full_unstemmed | Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title_short | Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment. |
| title_sort | quinolone resistant salmonella typhi in viet nam molecular basis of resistance and clinical response to treatment |
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