Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons.
Expression of cannabinoid 1 (CB1) and vanilloid 1 (VR1) receptor proteins was studied in adult, cultured rat dorsal root ganglion neurons. Immunostaining of CB1 receptors alone produced labelling in 57+/-2% of the cultured dorsal root ganglion neurons (n=3 cultures). The area of the labelled cells w...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1797105927765098496 |
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| author | Ahluwalia, J Urban, L Capogna, M Bevan, S Nagy, I |
| author_facet | Ahluwalia, J Urban, L Capogna, M Bevan, S Nagy, I |
| author_sort | Ahluwalia, J |
| collection | OXFORD |
| description | Expression of cannabinoid 1 (CB1) and vanilloid 1 (VR1) receptor proteins was studied in adult, cultured rat dorsal root ganglion neurons. Immunostaining of CB1 receptors alone produced labelling in 57+/-2% of the cultured dorsal root ganglion neurons (n=3 cultures). The area of the labelled cells was between 200 and 800 microm(2) with an average of 527+/-68 microm(2). VR1 immunolabelling revealed immunopositivity in 42+/-6% of the total population of dorsal root ganglion neurons. Cells showing VR1-like immunopositivity had an area between 200 and 600 microm(2). The mean area of the VR1-like immunopositive neurons was 376+/-61 microm(2). Double immunostaining with antisera raised against the CB1 and VR1 receptor proteins, showed a high degree of co-expression between CB1 and VR1 receptors. An average of 82+/-3% of the CB1-like immunopositive cells also showed VR1-like immunoreactivity (n=3 cultures) while 98+/-2% of the VR1-like immunolabelled neurons showed CB1 receptor-like immunostaining (n=3 cultures). Our data suggests that nociceptive primary sensory neurons co-express CB1 and VR1 receptors to a very high degree. We propose that this may provide an anatomical basis for a powerful combination of VR1 mediated excitation and CB1-mediated inhibition of nociceptive responses at central and peripheral terminals of nociceptive primary afferents. |
| first_indexed | 2024-03-07T06:54:22Z |
| format | Journal article |
| id | oxford-uuid:fd9f5bc3-e80d-4ee5-a591-1356cab293aa |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:54:22Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:fd9f5bc3-e80d-4ee5-a591-1356cab293aa2022-03-27T13:30:14ZCannabinoid 1 receptors are expressed in nociceptive primary sensory neurons.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fd9f5bc3-e80d-4ee5-a591-1356cab293aaEnglishSymplectic Elements at Oxford2000Ahluwalia, JUrban, LCapogna, MBevan, SNagy, IExpression of cannabinoid 1 (CB1) and vanilloid 1 (VR1) receptor proteins was studied in adult, cultured rat dorsal root ganglion neurons. Immunostaining of CB1 receptors alone produced labelling in 57+/-2% of the cultured dorsal root ganglion neurons (n=3 cultures). The area of the labelled cells was between 200 and 800 microm(2) with an average of 527+/-68 microm(2). VR1 immunolabelling revealed immunopositivity in 42+/-6% of the total population of dorsal root ganglion neurons. Cells showing VR1-like immunopositivity had an area between 200 and 600 microm(2). The mean area of the VR1-like immunopositive neurons was 376+/-61 microm(2). Double immunostaining with antisera raised against the CB1 and VR1 receptor proteins, showed a high degree of co-expression between CB1 and VR1 receptors. An average of 82+/-3% of the CB1-like immunopositive cells also showed VR1-like immunoreactivity (n=3 cultures) while 98+/-2% of the VR1-like immunolabelled neurons showed CB1 receptor-like immunostaining (n=3 cultures). Our data suggests that nociceptive primary sensory neurons co-express CB1 and VR1 receptors to a very high degree. We propose that this may provide an anatomical basis for a powerful combination of VR1 mediated excitation and CB1-mediated inhibition of nociceptive responses at central and peripheral terminals of nociceptive primary afferents. |
| spellingShingle | Ahluwalia, J Urban, L Capogna, M Bevan, S Nagy, I Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title | Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title_full | Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title_fullStr | Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title_full_unstemmed | Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title_short | Cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons. |
| title_sort | cannabinoid 1 receptors are expressed in nociceptive primary sensory neurons |
| work_keys_str_mv | AT ahluwaliaj cannabinoid1receptorsareexpressedinnociceptiveprimarysensoryneurons AT urbanl cannabinoid1receptorsareexpressedinnociceptiveprimarysensoryneurons AT capognam cannabinoid1receptorsareexpressedinnociceptiveprimarysensoryneurons AT bevans cannabinoid1receptorsareexpressedinnociceptiveprimarysensoryneurons AT nagyi cannabinoid1receptorsareexpressedinnociceptiveprimarysensoryneurons |