Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms.
A potent inhibitor of protein kinase C (PKC), inhibitor protein-1 (KCIP-1), isolated from sheep brain has been shown to consist of eight isoforms by reverse-phase HPLC. Direct protein sequence analysis has revealed these to be the same as those of 14-3-3 protein, described as an activator of tyrosin...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1992
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| _version_ | 1797105956478255104 |
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| author | Toker, A Sellers, L Amess, B Patel, Y Harris, A Aitken, A |
| author_facet | Toker, A Sellers, L Amess, B Patel, Y Harris, A Aitken, A |
| author_sort | Toker, A |
| collection | OXFORD |
| description | A potent inhibitor of protein kinase C (PKC), inhibitor protein-1 (KCIP-1), isolated from sheep brain has been shown to consist of eight isoforms by reverse-phase HPLC. Direct protein sequence analysis has revealed these to be the same as those of 14-3-3 protein, described as an activator of tyrosine and tryptophan hydroxylases involved in neurotransmitter biosynthesis. The N-termini of KCIP-1 isoforms were shown to be acetylated, and secondary structure predictions revealed a high degree of alpha-helix with an amphipathic nature. KCIP-1 showed no inhibitory activity towards protein kinase M (the catalytic fragment of PKC) and had no effect on the activities of three other protein kinases, cAMP-dependent protein kinase, Ca2+/calmodulin-dependent protein kinase II and casein kinase 2. Four forms of KCIP-1 were shown to be substrates for PKC in vitro, but none were phosphorylated by the other protein kinases mentioned above. |
| first_indexed | 2024-03-07T06:54:48Z |
| format | Journal article |
| id | oxford-uuid:fdc193dd-5ac4-49ab-b516-5b54924670b2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:54:48Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:fdc193dd-5ac4-49ab-b516-5b54924670b22022-03-27T13:31:17ZMultiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fdc193dd-5ac4-49ab-b516-5b54924670b2EnglishSymplectic Elements at Oxford1992Toker, ASellers, LAmess, BPatel, YHarris, AAitken, AA potent inhibitor of protein kinase C (PKC), inhibitor protein-1 (KCIP-1), isolated from sheep brain has been shown to consist of eight isoforms by reverse-phase HPLC. Direct protein sequence analysis has revealed these to be the same as those of 14-3-3 protein, described as an activator of tyrosine and tryptophan hydroxylases involved in neurotransmitter biosynthesis. The N-termini of KCIP-1 isoforms were shown to be acetylated, and secondary structure predictions revealed a high degree of alpha-helix with an amphipathic nature. KCIP-1 showed no inhibitory activity towards protein kinase M (the catalytic fragment of PKC) and had no effect on the activities of three other protein kinases, cAMP-dependent protein kinase, Ca2+/calmodulin-dependent protein kinase II and casein kinase 2. Four forms of KCIP-1 were shown to be substrates for PKC in vitro, but none were phosphorylated by the other protein kinases mentioned above. |
| spellingShingle | Toker, A Sellers, L Amess, B Patel, Y Harris, A Aitken, A Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title | Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title_full | Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title_fullStr | Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title_full_unstemmed | Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title_short | Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms. |
| title_sort | multiple isoforms of a protein kinase c inhibitor kcip 1 14 3 3 from sheep brain amino acid sequence of phosphorylated forms |
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