The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils
<p>Basophils are granulocytes involved in parasite immunity and allergic diseases, known for their potent secretion of type 2 cytokines. Identifying their functions has proven to be controversial due to their relative rarity and their complex lineage phenotype. Here, we show that the expressio...
| Asıl Yazarlar: | , , , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Frontiers Media
2019
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| _version_ | 1826310049329315840 |
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| author | Pellefigues, C Mehta, P Prout, MS Naidoo, K Yumnam, B Chandler, J Chappell, S Filbey, K Camberis, M Le Gros, G |
| author_facet | Pellefigues, C Mehta, P Prout, MS Naidoo, K Yumnam, B Chandler, J Chappell, S Filbey, K Camberis, M Le Gros, G |
| author_sort | Pellefigues, C |
| collection | OXFORD |
| description | <p>Basophils are granulocytes involved in parasite immunity and allergic diseases, known for their potent secretion of type 2 cytokines. Identifying their functions has proven to be controversial due to their relative rarity and their complex lineage phenotype. Here, we show that the expression of basophils lineage markers CD200R3 and FcεRIα is highly variable in inflammatory settings and hinders basophils identification by flow cytometry across multiple disease states or tissues. Fluorophore-conjugated antibody staining of these lineage markers strongly activates basophil type 2 cytokine expression, and represents a potential bias for coculture or <em>in vivo</em> transfer experiments. The Basoph8 is a mouse model where basophils specifically express a strong fluorescent reporter and the Cre recombinase. Basophils can be identified and FACS sorted unambiguously by their expression of the enhanced yellow fluorescent protein (eYFP) in these mice. We show that the expression of the eYFP is robust <em>in vivo</em> during inflammation, and in vitro on living basophils for at least 72 h, including during the induction of anaphylactoid degranulation. We bred and characterized the Basoph8xiDTR mice, in which basophils specifically express eYFP and the simian diphtheria toxin receptor (DTR). This model enables basophils conditional depletion relatively specifically <em>ex vivo</em> and <em>in vivo</em> during allergic inflammation and their detection as eYFP+ cells. In conclusion, we report underappreciated benefits of the commercially available Basoph8 mice to study basophils function.</p> |
| first_indexed | 2024-03-07T07:46:17Z |
| format | Journal article |
| id | oxford-uuid:fdc1f43e-65b0-464e-b58b-6e55ae876979 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:46:17Z |
| publishDate | 2019 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:fdc1f43e-65b0-464e-b58b-6e55ae8769792023-05-26T11:19:33ZThe Basoph8 mice enable an unbiased detection and a conditional depletion of basophilsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fdc1f43e-65b0-464e-b58b-6e55ae876979EnglishSymplectic ElementsFrontiers Media 2019Pellefigues, CMehta, PProut, MSNaidoo, KYumnam, BChandler, JChappell, SFilbey, KCamberis, MLe Gros, G<p>Basophils are granulocytes involved in parasite immunity and allergic diseases, known for their potent secretion of type 2 cytokines. Identifying their functions has proven to be controversial due to their relative rarity and their complex lineage phenotype. Here, we show that the expression of basophils lineage markers CD200R3 and FcεRIα is highly variable in inflammatory settings and hinders basophils identification by flow cytometry across multiple disease states or tissues. Fluorophore-conjugated antibody staining of these lineage markers strongly activates basophil type 2 cytokine expression, and represents a potential bias for coculture or <em>in vivo</em> transfer experiments. The Basoph8 is a mouse model where basophils specifically express a strong fluorescent reporter and the Cre recombinase. Basophils can be identified and FACS sorted unambiguously by their expression of the enhanced yellow fluorescent protein (eYFP) in these mice. We show that the expression of the eYFP is robust <em>in vivo</em> during inflammation, and in vitro on living basophils for at least 72 h, including during the induction of anaphylactoid degranulation. We bred and characterized the Basoph8xiDTR mice, in which basophils specifically express eYFP and the simian diphtheria toxin receptor (DTR). This model enables basophils conditional depletion relatively specifically <em>ex vivo</em> and <em>in vivo</em> during allergic inflammation and their detection as eYFP+ cells. In conclusion, we report underappreciated benefits of the commercially available Basoph8 mice to study basophils function.</p> |
| spellingShingle | Pellefigues, C Mehta, P Prout, MS Naidoo, K Yumnam, B Chandler, J Chappell, S Filbey, K Camberis, M Le Gros, G The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title | The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title_full | The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title_fullStr | The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title_full_unstemmed | The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title_short | The Basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
| title_sort | basoph8 mice enable an unbiased detection and a conditional depletion of basophils |
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